Sensitive ergotamine (cas 113-15-5) determination in pharmaceuticals and biological samples using cloud point preconcentration and spectrofluorimetric detection

Add time:08/27/2019    Source:sciencedirect.com

A new cloud point extraction (CPE) method for ergotamine (cas 113-15-5) analysis using fluorimetric detection is described. Ergotamine from an aqueous solution was preconcentrated into a smaller surfactant-rich phase using nonionic surfactant polyoxyethylene(7.5)nonylphenylether (PONPE 7.5). Differently from the conventional CPE procedure in which the resulting surfactant-rich phase is diluted by a fluidificant before its analysis, in this method the fluorescence measurements were carried out directly onto the undiluted surfactant-rich phase. The high viscosity provided by the undiluted surfactant rich phase greatly improved the fluorescence emission of ergotamine, leading to a total enhancement factor of 1325. This spectral advantage plus the preconcentration factor achieved, contributed to the method sensitivity allowing the ergotamine determination at trace level concentration. Under optimal experimental conditions, a linear calibration curve was obtained from 3.81 × 10−7 to 1.10 μg mL−1, with detection and quantification limits of 0.11 and 0.38 pg mL−1, respectively. The accuracy and versatility of the present methodology were proved by analyzing ergotamine in real samples of different natures such as pharmaceuticals, urine and saliva.

We also recommend Trading Suppliers and Manufacturers of ergotamine (cas 113-15-5). Pls Click Website Link as below: cas 113-15-5 suppliers

Prev:ergotamine (cas 113-15-5)
Next:Brief ReportsValvular Heart Disease Associated With ergotamine (cas 113-15-5))