Stabilization of structure in near-infrared fluorescent proteins by binding of biliverdin (cas 114-25-0) chromophore
-
Add time:08/28/2019 Source:sciencedirect.com
Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes and their mutants with different location of Cys residues, which able to bind a biliverdin (cas 114-25-0) chromophore, or without these Cys residues were studied using intrinsic tryptophan fluorescence, NIR fluorescence and circular dichroism. It was shown that a covalent binding of the biliverdin chromophore to a Cys residue via thioether group substantially stabilizes the spatial structure of NIR FPs. The stability of the protein structure and the chromophore association strength strongly depends on the location of Cys residues and decreases in the following order: a protein with Cys residues in both domains, a protein with Cys in PAS domains, and a protein with Cys in GAF domains. NIR FPs without Cys residues capable to covalently attach biliverdin have the lowest stability, comparable to NIR FP apoforms.
We also recommend Trading Suppliers and Manufacturers of biliverdin (cas 114-25-0). Pls Click Website Link as below: cas 114-25-0 suppliers
Prev:Reduced biliverdin (cas 114-25-0) reductase-A levels are associated with early alterations of insulin signaling in obesity
Next:Chiral recognition of bilirubin and biliverdin (cas 114-25-0) in liposomes and micelles) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Singlet oxygen oxidation products of biliverdin (cas 114-25-0) IXα dimethyl ester08/30/2019
- Chiral recognition of bilirubin and biliverdin (cas 114-25-0) in liposomes and micelles08/29/2019
- Reduced biliverdin (cas 114-25-0) reductase-A levels are associated with early alterations of insulin signaling in obesity08/27/2019
- Reviewbiliverdin (cas 114-25-0) reductase isozymes in metabolism08/26/2019
- Short CommunicationCrystal structure of a biliverdin (cas 114-25-0)-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation08/25/2019
- ReviewRedox Functions of Heme Oxygenase-1 and biliverdin (cas 114-25-0) Reductase in Diabetes08/24/2019
- l-Histidine, arachidonic acid, biliverdin (cas 114-25-0), and l-cysteine-glutathione disulfide as potential biomarkers for cured pulmonary tuberculosis08/23/2019
- Is it worth expending energy to convert biliverdin (cas 114-25-0) into bilirubin?08/22/2019
- Research paperModeling structure and excitation of biliverdin (cas 114-25-0)-binding domains in infrared fluorescent proteins08/21/2019