Regular ArticleEvaluation of Sodium 4-hydroxybenzoate (cas 114-63-6) as an Hydroxyl Radical Trap Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography with Electrochemical Detection
-
Add time:08/30/2019 Source:sciencedirect.com
Molecular and tissue damage induced by reactive oxygen species is a serious consequence of the production of free radicals in biological systems. Biological markers produced by reactions with hydroxyl radicals are useful indices of free radical processesin vivo.In this respect, hydroxylation of aromatic compounds such as salicylate (2-hydroxybenzoate) has been used extensively as a measure of hydroxyl radical formation. 4-Hydroxybenzoate will also trap hydroxyl radicals with fewer of the complications for which salicylate has been criticized. We describe two sensitive and specific methods using gas chromatography–ion trap mass spectrometry and high-performance liquid chromatography with electrochemical detection for a number of these aromatic marker compounds in biological fluids. The use of an ion trap mass spectrometer provides enhanced sensitivity along with full mass spectral identification of the compounds of interest. 4-Hydroxybenzoate and salicylate were compared as hydroxyl radical traps (i) by determining relative hydroxyl radical trapping efficienciesin vitro,(ii) by measuring individual dihydroxybenzoate isomers in rat serum following intraperitoneal injection of either 2- or 4-hydroxybenzoate, and (iii) by comparingin vivohydroxyl radical trapping using intrastriatal microdialysis in the rat. The techniques described have broad applications in the area of free radical biomedical research.
We also recommend Trading Suppliers and Manufacturers of Sodium 4-hydroxybenzoate (cas 114-63-6). Pls Click Website Link as below: cas 114-63-6 suppliers
Prev:Coupled mutual diffusion in aqueous sodium (salicylate + sodium chloride) solutions at 25 °C
Next:Pharmacokinetics of Sodium phenylacetate (cas 114-70-5) and sodium benzoate following intravenous administration as both a bolus and continuous infusion to healthy adult volunteers) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Coupled mutual diffusion in aqueous sodium (salicylate + sodium chloride) solutions at 25 °C08/29/2019
- Original ArticleAntioxidant activity and calcium binding of isomeric hydroxybenzoates08/28/2019
- Kinetics of the photosensitized degradation of benzyl 4-hydroxybenzoate in homogeneous aqueous solution under visible-light irradiation08/27/2019
- Stimulation of polyprenyl 4-hydroxybenzoate transferase activity by sodium cholate and 3-[(cholamidopropyl)dimethylammonio]-1-propanesulfonate08/26/2019
- Expression, purification and reconstitution of the 4-hydroxybenzoate transporter PcaK from Acinetobacter sp. ADP1☆08/25/2019
- Synthesis of 3,5-ditert-butyl-4-hydroxybenzoates and their thermal antioxidation behavior for polypropylene08/24/2019
- Esterification of Sodium 4-hydroxybenzoate (cas 114-63-6) by ultrasound-assisted solid–liquid phase-transfer catalysis using dual-site phase-transfer catalyst08/23/2019
- Rational engineering of the shikimate and related pathways in Corynebacterium glutamicum for 4-hydroxybenzoate production08/22/2019
-
Health and Chemical more >
-
Related Products
- Sodium 2,4-dimethylbenzenesulfonate
- SODIUM γ-FLUORO-β-HYDROXYBUTYRATE
- Sodium ((3-methoxy-1-methyl-3-oxo-1-propenyl)amino)phenylacetate
- Sodium (+)-10-camphorsulfonate
- Sodium (2-carbamoylphenoxy)acetate
- Sodium (2-methyl-4-chlorophenoxy)acetate
- Sodium (6R,7R)-3-[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanylmethyl]-8-oxo-7-[[2-(tetrazol-1-yl)acetyl]amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate pentahydrate
- Sodium (C10-16)alkylbenzenesulfonate
- Sodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)amino)propyl)-1,3-benzodioxole-2,2-
- Sodium (S)-lactate