Both A chain and B chain of β-bungarotoxin are functionally involved in the facilitation of spontaneous transmitter release in Xenopus nerve–muscle cultures
-
Add time:09/05/2019 Source:sciencedirect.com
In the present study, Xenopus nerve–muscle cultures were used to explore the functional roles of A chain (a phospholipase A2 subunit) and B chain (a non-phospholipase A2 subunit) of Bungarus multicinctus β-bungarotoxin. It was found that β-bungarotoxin induced an increment of the frequency of spontaneous synaptic currents (SSCs) in the nerve–muscle cultures. Modification of β-bungarotoxin with pyridoxal-5′-phosphate or substitution of Ca2+ with Ba2+ in buffer abolished the phospholipase A2 activity of β-bungarotoxin and the facilitatory phase of SSC as well. Antibodies that were directed specifically against A chain or B chain effectively inhibited phospholipase A2 activity, and as a consequence the SSC frequency was not greatly different from the control rate. These results suggest that both A and B chains are indispensable parts of β-bungarotoxin for inducing the facilitation of SSC frequency with Xenopus nerve–muscle cultures.
We also recommend Trading Suppliers and Manufacturers of BETA-BUNGAROTOXIN (cas 12778-32-4). Pls Click Website Link as below: cas 12778-32-4 suppliers
Prev:KChIP3: A binding protein for Taiwan banded krait β-bungarotoxin
Next:Aggregation of trypsin and TRYPSIN INHIBITOR (cas 12786-39-9) by Al cation) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- KChIP3: A binding protein for Taiwan banded krait β-bungarotoxin09/04/2019
- An ISFET-based immunosensor for the detection of β-Bungarotoxin09/03/2019
- Research paperβ-Bungarotoxin application to the round window: An in vivo deafferentation model of the inner ear09/02/2019
- Divergence of genes encoding B chains of β-bungarotoxins09/01/2019
- Mechanism of β-bungarotoxin in facilitating spontaneous transmitter release at neuromuscular synapse08/31/2019
- What does β-bungarotoxin do at the neuromuscular junction?08/30/2019
- Membrane-damaging activity with A chain and B chain of β-bungarotoxin08/29/2019
- Short communicationThe effect of temperature on the effects of the phospholipase A2 neurotoxins β-bungarotoxin and taipoxin at the neuromuscular junction08/28/2019
- β-bungarotoxin-induced depletion of synaptic vesicles at the mammalian neuromuscular junction08/27/2019