Metabolism of 2- and 3-TERT-BUTYL-4-HYDROXYANISOLE (cas 121-00-6) in the rat (III): Metabolites in the urine and feces

Add time:08/30/2019    Source:sciencedirect.com

The urinary and fecal metabolites of orally administered 2-tert-butyl-4-hydroxyanisole (2-BHA) and 3-TERT-BUTYL-4-HYDROXYANISOLE (cas 121-00-6) (3-BHA) in rats were identified. Samples of 2-day pooled urine and feces of rats given a single intragastric dose of 1 g/kg wt of tert[butyl-14C]3-BHA (∗Bu-3-BHA). tert[butyl-14C]2-BHA (∗Bu-2-BHA), [methyl-14C]3-BHA (∗Me-3-BHA) or [methyl-14C]-2-BHA (∗Me-2-BHA) were analyzed by comparing thin-layer chromatography (TLC) retentions with authentic standards. Conjugated metabolites were identified after enzymatic hydrolysis. Proton magnetic resonance spectroscopy and electron impact mass spectrometry were used for confirmation of the authentic standards. In rats given 3-BHA, a major metabolite in the urine was 3-BHA-glucuronide with a smaller amount of tert-butylhydroquinone (TBHQ)-sulfate, which unchanged 3-BHA and 3-BHA-glucuronide were detected in the feces. In rats given 2-BHA, the main metabolites were the sulfate conjugates of 2-BHA, 4-tert-butyl-5-methoxy-1,2-benzoquinone (2-TBOQ) and the glucoronide of 2-BHA in the urine, while unchanged 2-BHA was found in the feces.

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