Resistance of Rapistrum rugosum to tribenuron and Imazamox (cas 114311-32-9) due to Trp574 or Pro197 substitution in the acetolactate synthase

Add time:09/06/2019    Source:sciencedirect.com

Ten putative resistant and two susceptible Rapistrum rugosum populations originating from Greece were studied for resistance to acetolactate synthase (ALS)-inhibiting herbicides, using dose–response assays, sequencing of als gene and in vitro ALS activity assays. The dose–response assays showed that one (P1) out of ten putative resistant populations was cross-resistant to tribenuron and Imazamox (cas 114311-32-9), while another population (P4) was resistant to tribenuron only. All populations were susceptible to MCPA at the recommended rate. Gene sequencing of als revealed that the P4 population had a point mutation at Pro197 by His providing resistance to tribenuron, whereas the P1 had a Trp574 by Leu point mutation conferring cross-resistance to tribenuron and imazamox. The in vitro activity of the ALS enzyme indicated I50 values (tribenuron concentration required for 50% reduction of the ALS activity) ranging from 66.68 to 137.01 μM, whereas the respective value for the S populations ranged from 0.29 to 0.54 μM. These results strongly support that two R. rugosum populations evolved resistance to ALS-inhibiting herbicides due different point mutations in the als gene.

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