Development of a routine analysis of 4-Mercapto-4-methylpentan-2-one (cas 19872-52-7) in wine by stable isotope dilution assay and mass tandem spectrometry

Add time:10/01/2019    Source:infona.pl

The 4-Mercapto-4-methylpentan-2-one (cas 19872-52-7) (4MMP) is a key aroma compound in wines, especially in Sauvignon Blanc ones. Its accurate quantification is quite difficult due to its traces levels and its reactivity in wine conferred by the thiol function. In this paper, we proposed a new method for its quantification in wine without any sample preparation, based on automated derivatization procedure by methoximation and SIDA–SPME–GC–MS/MS analysis. The derivatization procedure was adapted from a previously published method in order to decrease the amount of reagents and the volume of wine (only 3mL are required). The use of SPME and the detection conditions have also been optimized to reach the best sensitivity as possible. The method was then validated according to the International Organization of Vine and Wine recommendations and exhibited excellent performances. Indeed, this method allowed us to quantify the 4MMP in wine at traces levels (LOD=0.19ngL −1 ) with reproducible results (RSD<15%) and a very good accuracy (recovery=102%).

We also recommend Trading Suppliers and Manufacturers of 4-Mercapto-4-methylpentan-2-one (cas 19872-52-7). Pls Click Website Link as below: cas 19872-52-7 suppliers

Prev:The yeast IRC7 gene encodes a β-lyase responsible for production of the varietal thiol 4-Mercapto-4-methylpentan-2-one (cas 19872-52-7) in wine
Next:Evidence for an alternative biogenetic pathway leading to 3-mercaptohexanol and 4-Mercapto-4-methylpentan-2-one (cas 19872-52-7) in wines)