In vitro and in vivo effect of 2,6-Di-tert-butyl-4-methylphenol (cas 128-37-0) as an antibiofilm agent against quorum sensing mediated biofilm formation of Vibrio spp.

Add time:09/28/2019    Source:infona.pl

This study unveils the in vitro and in vivo antibiofilm potential of 2,6-Di-tert-butyl-4-methylphenol (cas 128-37-0) (DTBMP) from Chroococcus turgidus against Vibrio spp. In the preliminary study, cell free culture supernatant (CFCS) of C. turgidus inhibited the violacein production in biomarker strain Chromobacterium violaceum and its mutant strain CV026 in a dose dependent manner. The effective biofilm inhibitory concentration (BIC) of pure compound DTBMP from C. turgidus was identified as 250 μg/ml concentration in tested Vibrio species. Furthermore, DTBMP proved to effectively inhibit the bioluminescence production in V. harveyi and other biofilm related virulence traits such as exopolysaccharides (EPS) production, hydrophobicity index, swimming and swarming motility at its BIC concentration in three major pathogenic vibrios: V. harveyi, V. parahaemolyticus and V. vulnificus. The antibiofilm potential of DTBMP was validated through light, confocal laser scanning and scanning electron microscopic analyses. In addition, the non-bactericidal effect of DTBMP was determined through growth curve and 2,3-bis (2-methyloxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Real-time PCR studies revealed the down-regulation of master quorum sensing (QS) regulator genes of V. harveyi such as luxR, luxS, luxP, luxQ and luxO on treatment with DTBMP. In vivo results confirmed that DTBMP augmented the survival rate of Litopenaeus vannamei larvae up to 75, 88 and 66% upon infection with V. harveyi, V. parahaemolyticus and V. vulnificus, respectively. The results of this study ascertain the promising effects of DTBMP as an antibiofilm agent, which could be positively explored to treat biofilm-associated vibrios infections in aquaculture.

We also recommend Trading Suppliers and Manufacturers of 2,6-Di-tert-butyl-4-methylphenol (cas 128-37-0). Pls Click Website Link as below: cas 128-37-0 suppliers

Prev:Reactions of Acenaphthenediimine Aluminum Hydride with 1,3-Dicyclohexylcarbodiimide and 2,6-Di-tert-butyl-4-methylphenol (cas 128-37-0)
Next:Structure, spectral and electrochemical properties of the 2,6-Di-tert-butylphenol (cas 128-39-2)-functionalized iron and cobalt(II) clathrochelates and their phenylsulfide analogs)