Pretreatment with the compound ASPERULOSIDE (cas 14259-45-1) decreases acute lung injury via inhibiting MAPK and NF-κB signaling in a murine model

Add time:07/16/2019    Source:sciencedirect.com

ASPERULOSIDE (cas 14259-45-1), an iridoid glycoside found in Herba Paederiae, is a component from traditional Chinese herbal medicine. In this study, we aimed to investigate the protective effects and potential mechanisms of asperuloside action on inflammatory responses in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells and an LPS-induced lung injury model. The pro-inflammatory cytokines and signaling pathways were measured by enzyme-linked immunosorbent assays (ELISA) and Western blotting to determine the effects of asperuloside. We found that asperuloside can significantly downregulate tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 levels in vitro and in vivo, and treatment with asperuloside significantly reduced the lung wet-to-dry weight, histological alterations and myeloperoxidase activity in a murine model of LPS-induced acute lung injury (ALI). In addition, Western blot analysis that pretreatment with asperuloside remarkably blunted the phosphorylation of inhibitor of nuclear factor kappa-B (IκBα), extracellular signal-related kinases 1 and 2 (ERK1/2), c-Jun. N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK) in LPS-stimulated inflammation. These results indicate that asperuloside exerts its anti-inflammatory effect in correlation with inhibition of a pro-inflammatory mediator through suppressing nuclear factor kappa-B (NF-κB) nuclear translocation and MAPK phosphorylation in a dose-dependent manner.

We also recommend Trading Suppliers and Manufacturers of ASPERULOSIDE (cas 14259-45-1). Pls Click Website Link as below: cas 14259-45-1 suppliers

Prev:Na2Co3[Fe(CN)6]2: A promising cathode material for lithium-ion and sodium-ion batteries
Next:Attenuation of isoproterenol-induced cardiotoxicity in rats by NARIRUTIN (cas 14259-46-2) rich fraction from grape fruit)