590-47-6 Usage
Description
Betaine monohydrate, also known as trimethylglycine, is a naturally occurring compound found in various plants and animals. It is a zwitterionic compound with both a positive and negative charge, which gives it unique properties and makes it a versatile molecule for various applications.
Uses
Used in Molecular Biology:
Betaine monohydrate is used as a buffer component in the preparation of KLA buffer for performing polymerase chain reaction (PCR). It helps maintain the stability and specificity of the PCR process, ensuring accurate amplification of target DNA sequences.
Used in Enzyme Kinetics Studies:
Betaine monohydrate is used as a research tool to study its effects on the Michaelis-Menten constants (Km) and maximum reaction velocity (Vmax) of human aldose reductase. This helps researchers understand the enzyme's behavior and its potential role in various biological processes.
Used in Cancer Research:
Betaine monohydrate is used in both in vitro and in vivo studies to investigate its effects on the growth of A549 lung cancer cells. This research aims to explore the potential therapeutic applications of betaine monohydrate in cancer treatment and prevention.
Check Digit Verification of cas no
The CAS Registry Mumber 590-47-6 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,9 and 0 respectively; the second part has 2 digits, 4 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 590-47:
(5*5)+(4*9)+(3*0)+(2*4)+(1*7)=76
76 % 10 = 6
So 590-47-6 is a valid CAS Registry Number.
InChI:InChI=1/C5H11NO2.H2O/c1-6(2,3)4-5(7)8;/h4H2,1-3H3;1H2/p-1
590-47-6Relevant articles and documents
Contents of lecithin and choline in crude drugs
Yamasaki,Kikuoka,Nishi,Kokusenya,Miyamoto,Matsuo,Sato
, p. 105 - 107 (2007/10/02)
The determination of lecithin and choline in crude drugs was established by a combination of high performance liquid chromatography (HPLC) with electrochemical detector (ECD) and enzyme reaction. Lecithin in crude drugs extracted with a mixture of chloroform-methanol (2:1) at room temperature was hydrolyzed by phospholipase D. The hydrolyzate was injected to HPLC, and choline was separated from impurities by reverse phase column. The choline was converted to betaine and hydrogen peroxide by passing through column packed with immobilized choline oxidase. This hydrogen peroxide was detected by ECD. The peak area of hydrogen peroxide derived from lecithin was proportional to the concentration of lecithin from 0.10 to 1.52 μg/ml. Choline in crude drugs was extracted with ethanol under reflux and determined under the same HPLC conditions as lecithin. The peak area of hydrogen peroxide derived from choline was proportional to the concentration of choline from 0.01 to 0.45 μg/ml. The contents of lecithin and choline in 31 kinds of crude drugs were determined by these established methods. The results showed that Cervi Parvum Cornu, Kokurozin, Foenigraeci Semen and Psoraleae Semen contained more lecithin than other crude drugs, while Angelicae Radix, Foenigraeci Semen, Psoraleae Semen, and especially Hippocampus were found to contain more choline than other crude drugs.