72088-94-9 Usage
Description
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein, commonly used to label biomolecules through a reaction involving the carboxyl group. It is membrane impermeant, making it suitable for studies of membrane permeability. 5(6)-Carboxyfluorescein exhibits excellent fluorescence with excitation/emission at 492/514 nm, respectively, closely matching the 488 nm spectral line of argon-ion lasers.
Uses
Used in Biomedical Research:
5(6)-Carboxyfluorescein is used as a popular probe for the determination of intracellular pH, helping researchers understand cellular processes and monitor pH changes within cells.
Used in Chemical Synthesis:
5(6)-FAM, a form of 5(6)-carboxyfluorescein, is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates. It also serves as a starting material for the synthesis of other fluorescein-derived reagents, contributing to the development of new fluorescent compounds for various applications.
Used in Analytical Techniques:
As a popular probe for determining intracellular pH, 5(6)-carboxyfluorescein is utilized in analytical techniques to measure and monitor pH levels within cells, providing valuable insights into cellular functions and responses to different stimuli.
Check Digit Verification of cas no
The CAS Registry Mumber 72088-94-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,2,0,8 and 8 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 72088-94:
(7*7)+(6*2)+(5*0)+(4*8)+(3*8)+(2*9)+(1*4)=139
139 % 10 = 9
So 72088-94-9 is a valid CAS Registry Number.
