77-86-1 Usage
Description
Tris Base, also known as Tris(hydroxymethyl)aminomethane, is an organic compound commonly used as a biological buffer in various applications. It is a strong base with a pKa value of around 8.1, making it suitable for maintaining stable pH levels in solutions. Tris Base is highly soluble in water and has low UV absorbance, making it ideal for use in biological research and industrial applications.
Uses
Used in Biotechnology and Molecular Biology:
Tris Base is used as a buffer component in various applications, such as:
1. In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis, providing a stable pH environment for DNA separation.
2. As a component of sample buffer for SDS-PAGE, ensuring proper protein denaturation and separation based on molecular weight.
3. In the preparation of IEC (ion-exchange chromatography) buffer, facilitating the separation of charged molecules based on their affinity to the ion-exchange matrix.
Used in Chemical Synthesis:
Tris Base is used as a starting material or intermediate in the synthesis of:
1. Surface-active agents, such as detergents and emulsifiers, due to its ability to form complexes with various compounds.
2. Vulcanization accelerators, which are used in the rubber industry to speed up the硫化 process and improve the properties of rubber products.
3. Pharmaceuticals, as Tris Base can be used as a building block for the synthesis of various drug molecules.
Used in Cosmetics and Personal Care:
Tris Base serves as an emulsifying agent in cosmetic creams and lotions, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, and cleaning compounds. It helps to stabilize the emulsion and improve the texture and feel of the final product.
Used in Environmental Applications:
Tris Base acts as an absorbent for acidic gases, helping to neutralize and remove harmful acidic compounds from the environment.
Used in Odor Control:
Tris Base, specifically tromethamine, can help mask odors in formulations, making it useful in creating more pleasant-smelling products.
Used in Protein Crystallography:
Tris Base is used for protein crystal growth under different pH conditions, allowing researchers to study the structure and function of proteins in detail.
Used in Nematode Research:
Tris buffer with low ionic strength can be used to study the formation of lamin intermediate fibers in the nematode C. elegans, providing insights into the molecular mechanisms of various biological processes.
Used in Surfactant, Vulcanization Accelerator, and Drug Synthesis:
Tris Base is a major component in the preparation of surfactants, vulcanization accelerators, and some drug intermediates, contributing to the development of various industrial and pharmaceutical products.
Used as a Standard in Titration:
Tris Base can also be used as a standard in titration, allowing for accurate determination of the concentration of other substances in a solution.
Uses of Biology
Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
Originator
Trisaminol,Bellon,France,1964
Preparation
Tris is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.
Manufacturing Process
Nitromethane is reacted with formaldehyde to give
tris(hydroxymethyl)nitromethane in an initial step. This intermediate may be
reduced by catalytic hydrogenation (US Patent 2,174,242) or by electrolytic
reduction (US Patent 2,485,982).
Biochem/physiol Actions
Trizma base, commonly known as tris is widely used as a biological buffer or as a component of buffer formulations like- Tris-EDTA (TE) buffer, Tris magnesium buffer or Tris-acetate-EDTA (TAE) buffer. It enhances the membrane permeability of cell membranes.
Purification Methods
TRIS can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]
Check Digit Verification of cas no
The CAS Registry Mumber 77-86-1 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 7 and 7 respectively; the second part has 2 digits, 8 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 77-86:
(4*7)+(3*7)+(2*8)+(1*6)=71
71 % 10 = 1
So 77-86-1 is a valid CAS Registry Number.
InChI:InChI:1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
77-86-1Relevant articles and documents
Segl'a, P.,Jamnicky, M.
, p. 221 - 230 (1993)
Sanger dideoxy sequencing of DNA
Walker, Sarah E.,Lorsch, Jon
, p. 171 - 184 (2013)
While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder.
COMPOSITION FOR STERILIZATION AND CLEANING, METHOD FOR PRODUCING SAME, AND STERILIZATION AND CLEANING METHOD EMPLOYING SAME
-
, (2022/01/08)
A composition for sterilization and cleaning which comprises 0.001-30 mass % peracetic acid, 0.001-30 mass % hydrogen peroxide, 1-70 mass % acetic acid and/or salt thereof, 0.001-5 mass % polyoxyethylene lauryl ether acetate or salt thereof, and water, the composition having a pH of 4.0-10.0.
Topical acne vulgaris composition with a sunscreen
-
, (2009/03/07)
Increased compliance in the use of topical sunscreens is obtained by combining a topical sunscreen agent in a formulation containing an antibacterial medication such as azelaic acid or an antibiotic.
