9001-60-9 Usage
Description
L-Lactic dehydrogenase, also known as LDH, is an enzyme that catalyzes the conversion of pyruvate to L-lactate in the presence of reduced coenzyme. It plays a crucial role in cellular metabolism and energy production.
Uses
Used in Clinical Diagnostics:
L-Lactic dehydrogenase is used as a diagnostic tool for various medical conditions, including myocardial infarction and leukemia. Its elevated levels in the blood can indicate tissue damage or disease progression.
Used in Cytotoxicity Assays:
L-Lactic dehydrogenase from bovine heart is used as a standard in cytotoxicity assays, which are essential for assessing the toxicity of substances on cells and evaluating the safety of new drugs or chemicals.
Used in Enzyme Activity Assays:
L-Lactic dehydrogenase is used in glutamic pyruvic transaminase (GPT) assay, which is a common test for liver function and damage. It is also used in ATPase assays of various proteins, such as the R2 complex Rvb1p-Rvb2p, RecA protein, and sarcoplasmic reticulum Ca2+-ATPase (SERCA), to study their enzymatic activities and functions.
Used in Research and Development:
L-Lactic dehydrogenase is used in research for studying cellular metabolism, energy production, and the regulation of various metabolic pathways. It is also employed in the development of new diagnostic tools and therapeutic agents for various diseases.
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Purification Methods
A forty-fold purification of the dehydrogenase is effected by affinity chromatography using Sepharose 4B coupled to 8-(6-aminohexyl)amino-5'-AMP or -NAD . [Lees et al. Arch Biochem Biophys 163 561 1974, Pesce et al. J Biol Chem 239 1753 1964.]
Check Digit Verification of cas no
The CAS Registry Mumber 9001-60-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,0 and 1 respectively; the second part has 2 digits, 6 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 9001-60:
(6*9)+(5*0)+(4*0)+(3*1)+(2*6)+(1*0)=69
69 % 10 = 9
So 9001-60-9 is a valid CAS Registry Number.
