9025-13-2 Usage
Description
EC 3.5.2.10, also known as creatinine amidohydrolase, is an enzyme that catalyzes the hydrolysis of creatinine to form sarcosine and urea. It plays a crucial role in the enzymatic determination of creatinine levels in clinical analysis.
Uses
Used in Clinical Analysis:
EC 3.5.2.10 is used as an enzymatic tool for the determination of creatinine levels in clinical analysis. It is coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase, and formaldehyde dehydrogenase to accurately measure creatinine concentrations, which are essential for assessing kidney function and diagnosing related disorders.
Biochem/physiol Actions
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Check Digit Verification of cas no
The CAS Registry Mumber 9025-13-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,2 and 5 respectively; the second part has 2 digits, 1 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 9025-13:
(6*9)+(5*0)+(4*2)+(3*5)+(2*1)+(1*3)=82
82 % 10 = 2
So 9025-13-2 is a valid CAS Registry Number.