9025-24-5 Usage
Description
Carboxypeptidase B is an enzyme that specifically cleaves C-terminal lysine and arginine residues from proteins and peptides. It is a zinc-dependent endopeptidase that plays a crucial role in the processing and regulation of peptide hormones and other biologically active molecules.
Uses
Used in Pharmaceutical Industry:
Carboxypeptidase B is used as a research tool for developing homogeneous time-resolved fluorescence (HTRF) assays to measure enzyme activity in a miniaturized high-throughput screening format. This allows for the evaluation of the impact of C-terminal lysine(s) in human plasminogen binding to Bifidobacterium and the measurement of the effect of treatment with carboxypeptidase B using flow cytometry analysis.
Used in Biomedical Research:
Carboxypeptidase B from Sigma is used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. It is also used to digest plasma samples by removing C-terminal basic amino acids, resulting in a distinct band for each allotype during C4 electrophoresis. This application aids in the study of protein processing and regulation in various biological systems.
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.
Check Digit Verification of cas no
The CAS Registry Mumber 9025-24-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,2 and 5 respectively; the second part has 2 digits, 2 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 9025-24:
(6*9)+(5*0)+(4*2)+(3*5)+(2*2)+(1*4)=85
85 % 10 = 5
So 9025-24-5 is a valid CAS Registry Number.
