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9030-66-4

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9030-66-4 Usage

Description

Glycerokinase, also known as glycerol kinase (glpK), is an enzyme that plays a crucial role in the metabolism of glycerol, a key component in the synthesis of triglycerides and phospholipids. It catalyzes the phosphorylation of glycerol to glycerol-3-phosphate (glycerol-3P) using adenosine triphosphate (ATP) as a cofactor. Glycerokinase is involved in various biological processes, including lipolysis, glycerol metabolism, and energy homeostasis.

Uses

Used in Clinical Analysis:
Glycerokinase is used as an enzymatic tool for the determination of glycerol and triglyceride levels when coupled with other enzymes such as glycerol-3-phosphate dehydrogenase (G-3-P DH), glycerol-3-phosphate oxidase (G-3-P oxidase), pyruvate kinase (PYK), lactate dehydrogenase (LCD), and lipoprotein lipase (LPL). This application aids in the diagnosis and monitoring of various metabolic disorders and lipid-related conditions.
Used in Pain Control Research:
Glycerokinase has been utilized in studying the effects of pain-controlling neuropeptides on human fat cell lipolysis. This research helps in understanding the underlying mechanisms of pain regulation and the role of glycerol metabolism in the process.
Used in Metabolism and Energy Homeostasis Studies:
Glycerokinase is used in the conversion of glycerol to glycerol-3 phosphate (glycerol-3P) in white adipose tissue (WAT) adipocyte samples by the label distribution method. This application aids in the investigation of glycerol metabolism and its role in energy homeostasis and lipid synthesis.
Used in Derivatization of Glycerol:
Glycerokinase, in conjunction with adenosine triphosphate (ATP), is used for the derivatization of glycerol to sn-glycerol-3 phosphate (glycerol-3P). This process is essential for the synthesis of various lipids and the study of their biological functions.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Purification Methods

Commercial enzyme has been dialysed against 2mM Hepes, 5mM dithiothreitol and 0.3mM EDTA, followed by several changes of 20mM Hepes and 5mM dithiothreitol prior to storage under N2 at -20o. [Knight & Cleland Biochemistry 28 5728 1989.] The enzyme from pigeon liver is purified by acid-precipitation (acetate buffer at pH 5.1), (NH4)2SO4 fractionation, heat treatment (60o/ 1hour), calcium phosphate gel filtration, a second (NH4)2SO4 fractionation, dialysis, elution of inert proteins and crystallisation. This is done by repeatedly extracting the precipitate from the last step with 0.05M sodium pyrophosphate (pH 7.5) containing 1mM EDTA and 0.2M (NH4)2SO4 is added. Careful addition of solid (NH4)2SO4 to this solution leads to crystallisation of the enzyme. Recrystallisation is repeated. The enzyme is activated by Mg2+ and Mn2+ ions and is most stable in solutions in the pH 4.5-5.5 range. The stability is greatly increased in the presence of glycerol. It has Km for glycerol of 60WM and for ATP 9WM in glycine buffer pH 9.8 and 25o. [Kennedy Methods Enzymol 5 476 1962.]

Check Digit Verification of cas no

The CAS Registry Mumber 9030-66-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,3 and 0 respectively; the second part has 2 digits, 6 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 9030-66:
(6*9)+(5*0)+(4*3)+(3*0)+(2*6)+(1*6)=84
84 % 10 = 4
So 9030-66-4 is a valid CAS Registry Number.

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