9075-68-7

Basic information

• Product Name: PULLULANASE
• Synonyms: pullulanase from bacillus sp.;pullulanase from klebsiella pneumoniae;PULLULANASE FROM BACILLUS SP., POWDER, ~ 1 U/MG;PULLULANASE FROM BACILLUS*ACIDOPULLULYTI;pullulanase from bacillus acidopullulyticus;PULLULANASE SUSPENSION, FROM KLEBSIELLA PNEUMONIAE;Amylopectin 6-gluconohydrolase, Limit dextrinase;PromozymeTM 400L, Pullulan 6-glucano-hydrolase
• CAS NO: 9075-68-7
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-983-9
• Mol File: 9075-68-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Density: 1.25 g/mL at 25 °C(lit.)
• Vapor Pressure: 0Pa at 25℃
• Storage Temp.: 2-8°C
• Water Solubility: 100g/L at 25℃
• CAS DataBase Reference: PULLULANASE(CAS DataBase Reference)
• NIST Chemistry Reference: PULLULANASE(9075-68-7)
• EPA Substance Registry System: PULLULANASE(9075-68-7)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 9075-68-7(Hazardous Substances Data)

Usage

Description

Pullulanase is an essential endo-type enzyme that degrades pullulan, a polysaccharide. It is synthesized as an extracellular, cell surface-anchored lipoprotein by certain microorganisms, such as Bacillus brevis and Klebsiella. Type I pullulanases specifically cleave α-1,6-glucosidic linkages in various polysaccharides, while type II pullulanases also target α-1,6-glucosidic linkages. This enzyme plays a significant role in the glucose and maltose syrup industries, as well as in the production of branched cyclodextrins and grain processing biotechnology.

Uses

Used in Glucose and Maltose Syrup Industries:
Pullulanase is used as an industrial enzyme in the glucose and maltose syrup industries. It works in combination with other enzymes, such as β-amylase and isoamylase, to produce maltose and oligosaccharides.
Used in the Production of Branched Cyclodextrins:
Pullulanase serves as a catalyst in the production of branched cyclodextrins, where it can catalyze the condensation reaction.
Used in Grain Processing Biotechnology:
Pullulanase is applied as a processing aid in grain processing biotechnology for the production of ethanol and sweeteners.
Used in Starch-Debranching:
Pullulanase, as a glucanase, degrades pullulan and is used for starch-debranching, which is essential for the production of various industrial products.
Used in Studying Polysaccharide Utilization:
Pullulanase is also utilized in research to study the utilization of polysaccharides, providing insights into their structure and function.

References

https://en.wikipedia.org/wiki/Pullulanase http://www.sigmaaldrich.com/catalog/product/sigma/e2412?lang=en®ion=US https://www.hindawi.com/journals/er/2012/921362/ http://www.amano-enzyme.co.jp/eng/enzyme/15.html http://www.ssjournals.com/index.php/ijbr/article/view/610

Biochem/physiol Actions

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.