- Substrate specificity and regioselectivity of Δ12 and ω3 fatty acid desaturases from Saccharomyces kluyveri
-
Δ12 and ω3 fatty acid desaturases are key enzymes in the synthesis of polyunsaturated fatty acids (PUFAs), which are important constituents of membrane glycerolipids and also precursors to signaling molecules in many organisms. In this study, we determined the substrate specificity and regioselectivity of the Δ12 and ω3 fatty acid desaturases from Saccharomyces kluyveri (Sk-FAD2 and Sk-FAD3). Based on heterologous expression in Saccharomyces cerevisiae, it was found that Sk-FAD2 converted C16-20 monounsaturated fatty acids to diunsaturated fatty acids by the introduction of a second double bond at the ν + 3 position, while Sk-FAD3 recognized the ω3 position of C18 and C20. Furthermore, fatty acid analysis of major phospholipids suggested that Sk-FAD2 and Sk-FAD3 have no strong substrate specificity toward the lipid polar head group or the sn-positions of fatty acyl groups in phospholipids.
- Oura, Takahiro,Kajiwara, Susumu
-
experimental part
p. 3174 - 3179
(2009/04/07)
-
- Synthesis of phosphatidylcholine having a very long chain polyunsaturated fatty acid
-
A phosphatidylcholine 9 bearing icosadienoyl and docosahexaenoyl groups at the 1-and 2-positions respectively was synthesized. The synthetic route involves carbon chain elongation of linoleic acid via malonic ester synthesis, preparation of lyso-phosphatidylcholine via lipase-catalyzed mono-acylation of 2-O-methoxyethoxymethylglycerol and phosphodiester synthesis, and finally DCC-mediated esterification.
- Haider, Syed Shabbir,Tanaka, Masatoshi,Alam, Md Khorshed,Nakajima, Shuhei,Baba, Naomichi,Shimizu, Sakayu
-
p. 175 - 176
(2007/10/03)
-
- SPECIFICITY OF THE ENZYME SYSTEM PRODUCING LONG CHAIN ALDEHYDES IN THE GREEN ALGA, ULVA PERTUSA
-
The greater part of the enzyme activity producing long chain aldehydes (LCA) such as (8Z)-heptadecanal, (8Z,11Z)-heptadecadienal, (8Z,11Z,14Z)-heptadecatrienal was found in the 4000 g precipitate of homogenates of the green alga, Ulva pertusa.The LCA-forming activity was readily solubilized from the precipitate by addition of 0.2percent Triton X-100.The solubilized LCA-forming activity showed an optimum pH in the range 8.5-9.5. α-Linolenic acid was a very good substrate, but γ-linolenic acid was a poor substrate for the activity.Linoleic acid was the best substrate of an entire series of (ζ6Z, ζ9Z)-dienoic acids, in which the chain length varied from C13 to C20.Key Word Index - Ulva pertusa; green alga; odour; aldehydes; fatty acids; substrate specificity.
- Kajiwara, Tadahiko,Yoshikawa, Hiroshi,Matsui, Kenji,Hatanaka, Akikazu,Kawai, Tetsuo
-
p. 407 - 410
(2007/10/02)
-