- Practical Enzymatic Synthesis of Adenosine 5'-O-(3-Thiotriphosphate) (ATP-γ-S)
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An enzymatic procedure for the synthesis of adenosine 5'-O-(3-thiotriphosphate) (ATP-γ-S) on a 50-mmol scale from dihydroxyacetone, sodium thiophosphate, ADP, and phosphoenol pyruvate is described.The synthesis uses polyacrylamide gel immobilized glycerokinase coupled to a pyruvate kinase catalyzed ATP cofactor regeneration system, and polyacrylamide gel immobilized triosephosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase, and phosphoglycerate kinase coupled to a lactate dehydrogenase catalyzed NAD cofactor regeneration system.The ATP-γ-S is purified by adsorption on Dowex 1 and isolated as the sodium or barium salts in ca. 90 percent purity.
- Abril, Obsidiana,Crans, Debbie C.,Whitesides, George M.
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p. 1360 - 1364
(2007/10/02)
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