- SINGLE-STEP SYNTHESIS OF KYOTORPHIN IN FROZEN SOLUTIONS BY CHYMOTRYPSIN
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A novel method of Kyotorphin (H-Tyr-Arg-OH) synthesis without protecting and deprotecting procedures is described - α-chymotrypsin-catalyzed aminolysis of H-Tyr-OEt by H-Arg-OH in frozen mixtures.In this one step process yields of the peptide product above 80percent have been obtained.
- Meos, Helle,Tougu, Vello,Haga, Mati,Aaviksaar, Aavo,Schuster, Matthias,Jakubke, Hans-Dieter
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p. 1559 - 1564
(2007/10/02)
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- Method for protecting guanidino group and restoring the same
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A guanidino group in an amino acid or a peptide can be protected with a specific group, i.e. pentamethylbenzensulfonyl, 2,4,6-trimethoxybenzenesulfonyl, 4-methoxy-2,3,5,6-tetramethylbenzenesulfonyl, 4-methoxy-2,6-dimethylbenzenesulfonyl or 4-methoxy-2,3,6-trimethylbenzenesulfonyl, and said group may easily be removed without affecting the amino acid or the peptide to be derived from the protected amino acid or peptide. Thus, the present invention is useful in the synthesis of peptides containing the guanidino group.
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