AT-2433 B1 (CAS NO.: ), with its systematic name of 5H-Indolo(2,3-a)pyrrolo(3,4-c)carbazole-5,7(6H)-dione, 12-(6-O-(2,4-dideoxy-4-(methylamino)pentopyranosyl)-4-O-methylhexopyranosyl)-12,13-dihydro-6-methyl-, could be produced through many synthetic methods.

Following is one of the synthesis routes:
3,4-Bis(3-indolyl)-1-methylpyrroline-2,5-dione (I) is cyclized by hydrogenation with H₂ over Pd/C in DMF, followed by a treatment with TFA, to afford the hexacyclic dione (II), which then is condensed with aminosugar (III) in refluxing methanol and dehydrogenated with DDQ in THF to afford the target glycoside.