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4.6. HPLC analysis on chiral stationary phase
The enantiomeric purity was determined by HPLC analysis on
Chiralcel AD-H column (250 ꢁ 5 mm), Daicel Chemical Industries,
Japan. Mobile phase, 3% 2-propanol in hexane. Flow rate, 0.5 mL/
min. Detection wavelength, 220 nm. Retention times (S)-2: 18.7;
(R)-2: 23.2 min. (R)-4-Oxocyclopent-2-enyl acetate: 14.8; (S)-4-
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4.7. Assignment of product configuration
The configuration of tert-butyldimethylsilyl derivative 3 was as-
signed as (S)- from the measurement of its specific rotation and
comparison with the reported value.43 By inference, the configura-
tion of the unreacted phenylacetyl derivative was assigned as (R).
4.8. Determination of the enzyme activity
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Penicillin G acylase activity was assayed from the hydrolysis of
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red at 100 rpm. The pH of the reaction medium was maintained
at 8.0 by continuous titration with 0.1 M NaOH for 10 min. The en-
zyme activity is expressed in units where one unit of enzyme is the
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4.9. Stability of immobilized penicillin G acylase
The enzyme stability in a suspension of the organic solvent was
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We thank the Department of Science and Technology and CSIR
New Delhi for financial support.
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