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Crystallographic data for structural analysis have been depos-
ited with the Cambridge Crystallographic Data Centre. Copies of
this information may be obtained free of charge from the CCDC
uk). There are disordered solvents in structures of 4, 5 and 7.
Attempts were made to model these disorders or split it into
two positions, but were unsuccessful. PLATON /SQUEZZE was
used to correct the data for the presence of disordered sol-
3
vent.[21] Potential solvent volumes of 673, 800 and 344 were
found for 4, 5 and 7, respectively. 269, 464 and 160 electrons
per unit cell of scattering were located in the void. The stoichiom-
etry of solvent was calculated to two THF and one dichlor-
methane (4), four hexane and four chloroform (5), two acetone
and two chloroform molecules per unit cell of 7. CCDC 865746
(for 3), 865748 (for 4) 865749 (for 5) 865747 (for 6) and 865750
(for 7) contain the supplementary crystallographic data for this
paper. These data can be obtained free of charge from the Cam-
Cytotoxic Studies
Studies were performed on the human T-lymphocytic leukemia
cells MOLT-4 obtained from the American Type Culture Collection
(USA) and HL-60 obtained from the European Collection (Porton
Down, Salisbury, UK). The cells were cultured in Iscove’s modified
Dulbecco’s medium supplemented with 20% fetal calf serum and
0.05% L-glutamine (all Sigma-Aldrich, USA) in a humidified incu-
bator at 37ꢂC and a controlled 5% CO2 atmosphere. The cell lines
in the maximal range of up to 20 passages were used for this
study. Cytotoxicity of compound 9 was evaluated by the WST-1
cell viability test (Roche, Germany) according to the
manufacturer’s instructions. The assay is based on the reduction
of WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-
1,3-benzene disulfonate) by viable cells. The reaction produces
a colored soluble formazan salt.[22] The absorbance at 440 nm
was measured using a multiplate reader (Tecan Infinite 200).
Compound 9 was dissolved in cultivation medium to the desired
concentrations. The cells were seeded in a 96-well plate, incu-
bated in 1–1000 mmol lꢀ1 solutions of compound 9 for 24 h, then
washed in pure media and incubated for 180 min in WST-1 solu-
tion. The same cells incubated in the cultivation media only were
used as the control. Absorbance data were normalized to 100%
cell viability for non-treated cells; half inhibiting concentration
(IC50), defined as the concentration of the drug reducing cell
viability by 50%, was obtained from the dose–response sigmoid
using Origin Pro (version 8, Microcal Software, Inc., Northampton,
MA, USA).
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Acknowledgements
The authors wish to thank the Grant Agency of the Czech Repub-
lic (project no. GA207/10/0215) and the Ministry of Education of
the Czech Republic for financial support.
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