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the unit at the 5-position of the deoxyuridine (dUiodTP > dUethTP
> dUthiTP > dUpyrTP). Furthermore, interestingly, dUthiTP
could be incorporated and extended well into long DNA for primer
extension and even for PCR. This multiple-thiophene-incorporated
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PCR product exhibited
a stable B-form DNA structure, as
32. Tasara, T.; Angerer, B.; Damond, M.; Winter, H.; Dörhöfer, S.;
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demonstrated using CD spectroscopy. dUthiTP also exhibited full-
length extension products even when using dUthiTP with 24
continuous oligo dA units as a template. Thus, we believe that
dUthiTP is a promising fluorescent nucleotide for diverse biological
applications that require multiple incorporation and extension
directly (i.e., without additional post-synthesis treatment) without
disruption of the B-form DNA structure.
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Acknowledgments
This study was undertaken with support from the Cooperative
Research Program for Agriculture Science
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