Structure elucidation of colibactin and its DNA cross-links
Source and publish data:
Science (2019)
Update date: 2022-08-04
Topics:
Authors:
Xue, Mengzhao
Kim, Chung Sub
Healy, Alan R.
Wernke, Kevin M.
Wang, Zhixun
Frischling, Madeline C.
Shine, Emilee E.
Wang, Weiwei
Herzon, Seth B.
Crawford, Jason M.
Article abstract of DOI:10.1126/science.aax2685
Colibactin is a complex secondary metabolite produced by some genotoxic gut Escherichia coli strains. The presence of colibactin-producing bacteria correlates with the frequency and severity of colorectal cancer in humans. However, because colibactin has not been isolated or structurally characterized, studying the physiological effects of colibactin-producing bacteria in the human gut has been difficult. We used a combination of genetics, isotope labeling, tandem mass spectrometry, and chemical synthesis to deduce the structure of colibactin. Our structural assignment accounts for all known biosynthetic and cell biology data and suggests roles for the final unaccounted enzymes in the colibactin gene cluster.
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Full text of DOI:10.1126/science.aax2685
Cite as: M. Xue et al ., Science
10.1126/science.aax2685 (2019).
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Structure elucidation of colibactin and its DNA cross-links
Mengzhao Xue, Chung Sub Kim, Alan R. Healy, Kevin M. Wernke, Zhixun Wang, Madeline C. Frischling, Emilee E. Shine, Weiwei
Wang, Seth B. Herzon and Jason M. Crawford
published online August 8, 2019originally published online August 8, 2019
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