Analytical Chemistry p. 1861 - 1867 (1991)
Update date:2022-08-06
Topics:
Zhang, Renen
Xie, Zemin
Zhao, Rui
Li, Xiaohua
Liu, Guoquan
Aguilar, Marie-Isabel
Hearn, Milton T. W.
A new silane, di-n-butyltetramethyldisilazane (DBTMDS), has been synthesized. Silanizations withh DBTMDS of a narrow-pore and a wide-pore silica yielded relatively high concentrations of bonded n-butyl ligands, of 4.22 and 4.09 μmol/m2, respectively. The chromatographic properties of the n-butyl bonded phase (C4-NH), synthesized with DBTMDS, were compared with n-butyl bonded phases (C4-CL) synthesized with n-butyldimethylchlorosilane (BDMCS) and Vydac-C4 under the same chromatographic conditions. The adsorption of basic compounds including N,N-diethylaniline (N,N-DEA), dansylarginine (Dns-Arg), and (phenyithio)hydantoin-arginine (PTH-Arg), as well as several polypeptides and small proteins including angiotensin I, angiotensin II, and lysozyme, was shown to be less for the C4-NH sorbents than for C4-CL and Vydac-C4 sorbents. While the S value for the basic protein lysozyme was significantly lower on the wide-pore C4-NH sorbent, the S and log k0 values for a range of selected proteins were comparable to those obtained with the Vydac-C4 sorbent. The tests of hydrolytic stability of the bonded phases showed significantly greater stability for the C4-NH sorbents than for the C4-CL sorbents under isocratic mobile-phase conditions of 10% and 40% acetonitrile with 0.1% TFA.
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