10.1002/anie.202001302
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In conclusion, we have designed a scalable platform to
power enzymatic cascades with electricity. We furthermore
demonstrated the potential of the novel design by producing
various piperidine derivates from diamines. We extended the
applicability of the system towards performing regioselective
isotopic labeling and providing useful insights into imine reduction
by IREDs. This platform represents an important advance in the
field for biocatalytic synthesis, and it can be expanded for
powering various cofactor-dependent oxidoreductases.
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Conflict of interest:
There are no conflicts to declare.
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Schumacher, J. Mol. Catal. B: Enzym. 2013, 97, 169-174.
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Acknowledgements
A.A-S. and L.L. received funding from the German
Research Foundation (Deutsche Forschungsgemeinschaft, DFG,
project number 284111627) and the Einstein foundation. L. L. was
funded by the Deutsche Forschungsgemeinschaft (DFG, German
Research Foundation) under Germany´s Excellence Strategy –
EXC 2008/1 – 390540038) and by the Fonds der Chemischen
Industrie. U.-P. A. and K.j.P received funding from DFG (Emmy
[12]
[13]
[14]
Noether Grant to U.-P. A., AP242/2-1 and under Germany s
́
Excellence Strategy – EXC-2033 – Projektnummer 390677874) as
well as by the Fraunhofer Internal Programs under Grant No. Attract
097-602175. We thank Reinhard Schömäcker, Gabrielle Vetter,
Sebastian Kemper and Marc Griffel product analysis by GC, MS
and NMR, respectively, Oliver Lenz for generous support by using
his lab equipment, Changzhu Wu for providing Amberlite FPA 54
and Enginzyme (Stockholm, Sweden) for EziGTM
.
Keywords: Isotopic labelling • Electrochemical biocatalysis •
Imine reductase • Hydrogenase, N-heterocycles • Immobilisation
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