992
F. Corelli et al. / IL FARMACO 59 (2004) 987–992
tion of an amount of enzyme able to transform approximately
0% of thymidine present in the assay. The mixtures were
incubated for 15 min at 37 °C; 20 µl of the reaction mixtures
was then analyzed by HPLC in order to separate thymine
from thymidine. IC50 values were calculated by analysis of
the areas of the peaks eluted from the column.
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10] M. Botta, F. Corelli, G. Maga, F. Manetti, M. Renzulli, S. Spadari,
Research on anti-HIV-1 agents. Part 2: Solid-phase synthesis, biologi-
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One of us (S.P.) thanks SienaBiotech S.p.A., Siena (Italy),
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4
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