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The amount of 1-butanol was varied to give different ratios of
ethyl benzoate to butanol (1 : 1.2, 1 : 1.5, 1 : 2). The trials were
performed at 60 1C. A 15 mL sample was automatically trans-
ferred from the reaction vial to a vial containing acetonitrile (1
mL). The contents of the transfer vial were stirred for 1 min
before analysis by HPLC. The chromatography and data analy-
sis were performed in an analogous method to that use in the
case of enzyme loading trials.
10 L. M. Stencel, C. M. Kormos, K. B. Avery and
N. E. Leadbeater, Org. Biomol. Chem., 2009, 7, 2452.
11 There are a number of online tools for predicting potential
cleavage sites cleaved by proteases or chemicals in a given
peptide_cutter/ (Accessed September 30, 2013).
12 K. Faber, Biotransformations in Organic Chemistry: A Text-
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Varying the temperature for the reaction of ethyl benzoate and
1-butanol. In a 2 mL capacity HPLC vial, lipase (75 mg), decane
(1 mL) and ethyl benzoate (29 mL, 0.2 mmol) were combined.
When the HPLC was started, 1-butanol (27 mL, 0.3 mmol) was
added to the reaction vial. Trials were performed at 40–70 1C. A
15 mL sample was automatically transferred from the reaction
vial to a vial containing acetonitrile (1 mL). The contents of the
transfer vial were stirred for 1 min before analysis by HPLC. The
chromatography and data analysis were performed in an ana-
logous method to that use in the case of enzyme loading trials.
´
13 For an introduction, see: (a) V. Gotor-Fernandez and
V. Vicente, Use of Lipases in Organic Synthesis, in Industrial
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Acknowledgements
Reaction Analytics Inc. is thanked for access to an iChemEx-
plorer system and funding from the National Science Founda-
tion (CAREER award CHE-0847262) is acknowledged. Michael
Lopez of Reaction Analytics Inc. is thanked for training and
equipment support.
´
19 For specific examples, see: (a) P. Picotti, M. Clement-Ziza,
¨
H. Lam, D. S. Campbell, A. Schmidt, E. W. Deutsch, H. Rost,
Z. Sun, O. Rinner, L. Reiter, Q. Shen, J. J. Michaelson, A. Frei,
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2 (a) A. Sickmann, Practical Proteomics, Wiley, 2013;
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3 Enzyme Catalysis in Organic Synthesis, ed. K. Drauz,
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20 See, for example: (a) M. Salim, S. L. McArthur,
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5 V. R. Meyer, Practical High-Performance Liquid Chromatogra-
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