Bioscience, Biotechnology and Biochemistry p. 735 - 739 (1998)
Update date:2022-08-11
Topics:
Doi, Junko
Yokoigawa, Kumio
Isobe, Yuka
Kawai, Hiroyasu
We tried a method for the production of trehalose 6-phosphate (T6P) with energy-coupling fermentation by baker's yeast. T6P was produced in a reaction mixture containing glucose, 5′-UMP, MgSO4, inorganic phosphate, and dried cells of baker's yeast as the enzyme preparation. T6P was isolated from the reaction mixture and identified by TLC, HPLC, GC-MS, and enzymatic methods. The reaction conditions suitable for T6P production were investigated. The formation of T6P and its precursors, glucose 6-phosphate and UDPglucose, at various pHs and concentrations of substrates was examined. Accumulation of T6P was maximum with a reaction mixture containing 1 M glucose, 20 mM 5′-UMP, 20 mM MgSO4, 400 mM sodium phosphate buffer (pH 6.2), and 100 mg/ml dried cells of baker's yeast shaken at 37°C for 6 h. The yield of T6P as a percentage of glucose was 11% (mol/mol) under these reaction conditions.
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