JOURNAL OF THE CHINESE
CHEMICAL SOCIETY
Antioxidant Activity and Luminescence of a Mn(III) Complex
Antioxidation study methods: The hydroxyl radicals
were generated in aqueous media through the Fenton-type reac-
tion.45,46 The 3 mL reaction mixtures contained 1.0 mL of 40
mg/mL aqueous safranin, 1 mL of 1.0 mmol aqueous EDTA-
Fe(II), 1 mL of 3% aqueous H2O2, and a series of quantitatively
microadding solutions of the tested compound. The sample with-
out the tested compound was used as the control. The reaction
mixtures were incubated at 37 °C for 30 min in a water-bath.
Absorbance at 520 nm was measured and the solvent effect was
corrected throughout. The absorbance was then measured at 520
nm. The scavenging effect for OH· was calculated from the fol-
lowing expression:47
ACKNOWLEDGEMENTS
The present research was supported by the National
Natural Science Foundation of China (Grant No.
21367017), the Fundamental Research Funds for the
Gansu Province Universities (212086), National Natural
Science Foundation of Gansu Province (Grant No.
1212RJZA037), and ‘Qing Lan’ Talent Engineering Funds
for Lanzhou Jiaotong University.
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CONCLUSIONS
In conclusion, a Schiff base ligand bis(5-methyl-
salicylaldehyde)-3-oxapentane-1,5-diamine (H2L) and its
Mn(III) complex have been synthesized, structurally char-
acterized and properties. In addition, the Mn(III) complex
exhibited potential antioxidant activities against HO· and
O2- radicals in vitro studies. These findings indicate that
the Mn(III) complex has many potential practical applica-
tions for the development of nucleic acid molecular probes
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J. Chin. Chem. Soc. 2015, 62, 1028-1034
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