Biochemistry
Article
Funding
(12) Emsley, P., and Cowtan, K. (2004) Coot: model-building tools
for molecular graphics. Acta Crystallogr. D60, 2126−2132.
(13) Brunger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L.,
Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M.,
Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L.
1998) Crystallography & NMR system: A new software suite for
macromolecular structure determination. Acta Crystallogr., Sect. D: Biol.
Crystallogr. 54, 905−921.
This work was supported by National Institute of Health grant
U01 GM61388 (Y.P., V.C.Y.). Argonne is operated by
UChicago Argonne, LLC, for the U.S. Department of Energy,
Office of Biological and Environmental Research under
Contract DE-AC02-06CH11357
(
ACKNOWLEDGMENTS
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(14) Murshudov, G. N., Vagin, A. A., and Dodson, E. J. (1997)
We thank S. Ginell for beamline support. Diffraction data were
measured at the Structural Biology Center beamline 19-ID at
the Advanced Photon Source, Argonne National Laboratory.
Refinement of macromolecular structures by the maximum-likelihood
method. Acta Crystallogr. D53, 240−255.
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Thornton, J. M. (1993) PROCHECK: a program to check the
stereochemical quality of protein structures. J. Appl. Crystallogr. 26,
ABBREVIATIONS
■
2
83−291.
17) Balducci, E., Emanuelli, M., Raffaelli, N., Ruggieri, S., Amici, A.,
NNMT, nicotinamide N-methyltransferase; wt-hNNMT, wild-
type human NNMT; tm-hNNMT, hNNMT K100A/E101A/
E103A triple mutant; AdoMet, S-adenosyl-L-methionine;
AdoHcy, S-adenosyl-L-homocysteine; NCA, nicotinamide;
MTase, AdoMet-dependent methyltransferase; INMT, indole-
thylamine N-methyltransferase; PNMT, phenylethanolamine
N-methyltransferase; DXMT, 3,7-dimethylxanthine methyl-
transferase.
(
(
Magni, G., Orsomando, G., Polzonetti, V., and Natalini, P. (1995)
Assay methods for nicotinamide mononucleotide adenylyltransferase
of wide applicability. Anal. Biochem. 228, 64−68.
(18) van der Spoel, D., Lindahl, E., Hess, B., Groenhof, G., Mark, A.
E., and Berendsen, J. J. C. (2005) GROMACS: Fast, flexible, and free.
J. Comput. Chem. 26, 1701−1718.
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P. H., Kruger, P., Mark, A. E., Scott, W. R. P., and Tironi, I. G. (1996)
Biomolecular Simulation: the GROMOS96 Manual and User Guide,
Hochschulverlag AG an der ETZ Zurich, Zurich.
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dx.doi.org/10.1021/bi2007614|Biochemistry 2011, 50, 7800−7808