chelator upon reaction with H O . Further investigation on
structural modification of BSIH is needed to obtain new
derivatives that match the low inherent cytotoxicity of BSIH, yet
improved hydrolytic stability in aqueous buffered systems and
cell culture media.
2
2
1
20%
00%
1
80%
60%
40%
20%
0%
5
10 25 50 75 100
5
10 25 50 75 100
5
10 25 50 75 100
5
10 25 50 75 100
5
10 25 50 75 100
Ctrl H O2
BSIH (μM)
INH (μM)
FBA (μM)
SIH (μM)
SAL (μM)
2
Figure 6. Cytoprotection of BSIH-related components against H
concentrations of tested compounds for 5 h prior to exposure to H
percentage of the untreated control group (100%).
2
O
2
in ARPE-19 human retinal epithelial cells. ARPE-19 cells were pre-incubated with various
2
(200 μM). Cell viability was measured 19 h after peroxide treatment and expressed as
2
O
2
2
0. Oliveri, V.; Vecchio, G. J Inorg Biochem 2016, 162, 31.
1. Zheng, H.; Youdim, M. B.; Fridkin, M. ACS Chem Biol 2010, 5,
6
03.
Acknowledgments
2
2
2
2
2. Akam, E. A.; Chang, T. M.; Astashkin, A. V.; Tomat, E.
Metallomics 2014, 6, 1905.
3. Mertens, C.; Akam, E. A.; Rehwald, C.; Brüne, B.; Tomat, E.;
Jung, M. PLOS ONE 2016, 11, e0166164.
We thank the National Institutes of Health (RO1-GM084176)
for supporting this work.
4. Thiele, N. A.; Abboud, K. A.; Sloan, K. B. Eur J Med Chem
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