Bioscience, Biotechnology and Biochemistry p. 1702 - 1706 (2000)
Update date:2022-08-11
Topics:
Kiso, Taro
Nakano, Hirofumi
Nakajima, Hirofumi
Terai, Tadamasa
Okamoto, Katsuyuki
Kitahata, Sumio
p-Hydroxybenzoyl β-galactose (pHB-Gal) was synthesized chemically to examine the hydrolytic activity of β-galactosyl ester linkage by β-galactosidases. The enzyme from Penicillium multicolor hydrolyzed the substrate as fast as p-nitrophenyl β-galactoside (pNP-Gal), a usual substrate with a β-galactosidic linkage. The enzymes from Escherichia coli and Aspergillus oryzae hydrolyzed pHB-Gal with almost the same rates as pNP-Gal. The enzymes from Bacillus circulans, Saccharomyces fragilis, and bovine liver showed much lower activities. pH-activity profiles, inhibition analysis, and kinetic properties of the enzymic reaction on pHB-Gal suggested that β-galactosidase had only one active site for hydrolysis of both galactosyl ester and galactoside. The Penicillium enzyme hydrolyzed pHB-Gal in the presence of H218O to liberate galactose containing 18O. This result suggests the degradation occurs between the anomeric carbon and an adjacent O atom in the ester linkage of pHB-Gal.
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Doi:10.1016/S0040-4020(00)00445-2
(2000)Doi:10.1021/ja01022a068
(1968)Doi:10.1002/anie.200501860
(2005)Doi:10.1016/j.bmcl.2007.05.073
(2007)Doi:10.1002/chem.201201874
(2012)Doi:10.1063/1.462776
(1992)