2284
M. TAZOE et al.
6) Lam, H.-M., and Winkler, M. E., Metabolic relationships
derived antimetabolites with the enzymes of the threo-
nine biosynthetic pathway of Escherichia coli. J. Biol.
Chem., 259, 15331–15339 (1984).
between pyridoxine (vitamin B6) and serine biosynthesis
in Escherichia coli K-12. J. Bacteriol., 172, 6518–6528
(1990).
17) Farrington, G. K., Kumar, A., Shames, S. L.,
Ewaskiewicz, J. I., Ash, D. E., and Wedle, F. C.,
Threonine synthase of Escherichia coli: inhibition by
classical and slow-binding analogues of homoserine
phosphate. Arch. Biochem. Biophys., 307, 165–174
(1993).
18) Stock, A., Ortanderl, F., and Pfleiderer, G., Darstellung
von radioakiv markiertem pyridoxal 50-phosphat. Bio-
chem. Z., 344, 353–360 (1966).
19) Glenn, A. R., and Dilworth, M. J., The effect of metal
ions on the alkaline phosphatase of Rhizobium legumi-
nosarum. Arch. Microbiol., 126, 251–256 (1980).
20) Deng, S., Summers, M. L., Kahn, M. L., and McDermott,
T. R., Cloning and characterization of a Rhizobium
meliloti nonspecific acid phosphatase. Arch. Microbiol.,
170, 18–26 (1998).
21) Geladopoulos, T. P., Sotiroudis, T. G., and
Evangelopoulos, A. E., A malachite green colorimetric
assay for protein phosphatase activity. Anal. Biochem.,
192, 112–116 (1991).
7) Lam, H.-M., and Winkler, M. E., Characterization of
the complex pdxH-tyrS operon of Escherichia coli K-12
and pleiotropic phenotypes caused by pdxH insertion
mutants. J. Bacteriol., 174, 6033–6045 (1992).
8) Hill, R. E., Sayer, B. G., and Spenser, I. D., Biosynthesis
of vitamin B6: incorporation of D-1-deoxyxylulose.
J. Am. Chem. Soc., 111, 1916–1917 (1989).
9) Laber, B., Maurer, W., Scharf, S., Stepusin, K., and
Schmidt, F. S., Vitamin B6 biosynthesis: formation of
pyridoxine 50-phosphate from 4-(phosphohydroxy)-L-
threonine and 1-deoxy-D-xylulose 5-phosphate by PdxA
and PdxJ protein. FEBS Lett., 449, 45–48 (1999).
10) Zhao, G., and Winkler, M. E., Kinetic limitation and
cellular amount of pyridoxine (pyridoxamine) 50-phos-
phate oxidase of Escherichia coli K-12. J. Bacteriol.,
177, 883–891 (1995).
11) Tazoe, M., Ichikawa, K., and Hoshino, T., Production of
vitamin B6 in Rhizobium. Biosci. Biotechnol. Biochem.,
63, 1378–1382 (1999).
12) Tazoe, M., Ichikawa, K., and Hoshino, T., Biosynthesis
of vitamin B6 in Rhizobium. J. Biol. Chem., 275, 11300–
11305 (2000).
22) Laemmli, U. K., Cleavage of structural proteins during
the assembly of the band of bacteriophage T4. Nature,
227, 680–685 (1970).
13) Tazoe, M., Ichikawa, K., and Hoshino, T., Biosynthesis
of vitamin B6 in Rhizobium: in vitro synthesis of
pyridoxine from 1-deoxy-D-xylulose and 4-hydroxy-L-
threonine. Biosci. Biotechnol. Biochem., 66, 934–936
(2002).
14) Capela, D., Barloy-Hubler, F., Gouzy, J., Bothe, G.,
Ampe, F., Batut, J., Boistard, P., Becker, A., Boutry, M.,
Cadieu, E., Dreano, S., Gloux, S., Godrie, T., Goffeau,
A., Kahn, D., Kiss, E., Lelaure, V., Masuy, D., Pohl, T.,
Portetelle, D., Puehler, A., Purnelle, B., Ramsperger, U.,
Renard, C., Thebault, P., Vandenbol, M., Weidner, S.,
and Galibert, F., Analysis of the chromosome sequence
of the legume symbiont Sinorhizobium meliloti strain
1021. Proc. Natl. Acad. Sci. U.S.A., 98, 9877–9882
(2001).
23) Matsudaira, P., Sequence from picomole quantities of
proteins electroblotted onto polyvinylidene difluoride
membranes. J. Biol. Chem., 232, 10035–10038 (1987).
24) Fonda, M. L., Purification and characterization of
vitamin B6-phosphate phosphatase from human erythro-
cytes. J. Biol. Chem., 267, 15978–15983 (1992).
25) Doi, M., and Shioi, Y., A novel phosphatase specific for
pyridoxal 50-phosphate in an aerobic photosynthetic
bacterium, Erythrobacter sp. OCh 114. FEMS Micro-
biol. Lett., 55, 309–313 (1988).
26) Gao, G. J., and Fonda, M. L., Identification of an
essential cysteine residue in pyridoxal phosphatase from
human erythrocytes. J. Biol. Chem., 269, 8234–8239
(1994).
27) Gao, G. J., and Fonda, M. L., Kinetic analysis and
chemical modification of vitamin B6 phosphatase from
human erythrocytes. J. Biol. Chem., 269, 7163–7168
(1994).
15) Wungsintaweekul, J., Herz, S., Hecht, S., Wisenreich,
W., Feicht, R., Rordich, F., Bacher, A., and Zenk, M. H.,
Phosphorylation of 1-deoxy-D-xylulose by D-xyluloki-
nase of Escherichia coli. Eur. J. Biochem., 268, 310–316
(2001).
28) Jang, Y. M., Kim, D. W., Kang, T.-C., Won, M. H.,
Baek, N.-I., Moon, B. J., Choi, S. Y., and Kwon, O.-S.,
Human pyridoxal phosphatase. J. Biol. Chem., 278,
50040–50046 (2003).
16) Shames, S. L., Ash, D. E., Wedler, F. C., and
Villafranca, J. J., Interaction of aspartate and aspartate-