Further Optimization

Synthesis Characterization Kinetic studies Synthetic route Purification Data Analysis Structure-Activity Relationship (SAR) Analysis Purification and characterization Chemical Synthesis In vitro Testing Biological Assays Animal Studies Structure-Activity Relationship (SAR) Studies Clinical Trials Formulation and Delivery Literature Review Biological Evaluation Mechanism of Action Publication and Patenting Regulatory Considerations Fluorescence Spectroscopy Photophysical Properties Lead Compound Selection Collaboration Design and Planning Preclinical Studies Design and Synthesis Background and Rationale Synthesis and Testing Toxicity and Safety Toxicity and Safety Evaluation Animal Studies (if required) Publication and Reporting Patent Application Understanding the Enzyme Mechanism Identifying a Suitable Transition State Analogue Molecular Modeling and Computational Chemistry Designing the Inhibitor Biochemical Assays Toxicity and Specificity Testing Preclinical Testing Scale-Up Synthesis Antibacterial Activity Testing Determination of Minimum Inhibitory Concentration (MIC) Combination Studies Publication and Dissemination Safety and Ethical Considerations Patent Consideration Biological Activity Testing Cell-Based Assays