1135-40-6 Usage
Description
N-Cyclohexyl-3-aminopropanesulfonic acid, commonly known as CAPS, is a zwitterionic buffering agent with a high water solubility. It is a white/clear crystalline powder that exhibits minimal reactivity with enzymes or proteins and has minimal salt effects. CAPS has an optimum buffering pH of 10.4, making it a valuable compound in various applications.
Uses
Used in Biological Buffers:
N-Cyclohexyl-3-aminopropanesulfonic acid is used as a biological buffer for maintaining a stable pH environment in various biochemical processes and experiments.
Used in Western and Immunoblotting Experiments:
CAPS is used as a buffer salt to formulate CAPS buffer, which is widely utilized in Western and immunoblotting experiments. The high pH of this buffer makes it suitable for the transfer of proteins with a pI > 8.5.
Used in Protein Sequencing and Identification:
N-Cyclohexyl-3-aminopropanesulfonic acid is used as a buffer in protein sequencing and identification processes, providing a stable pH environment that is crucial for accurate results.
Used in Electrotransfer of Proteins:
CAPS buffer is employed in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724), facilitating the efficient transfer of proteins during these procedures.
Flammability and Explosibility
Notclassified
Check Digit Verification of cas no
The CAS Registry Mumber 1135-40-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,1,3 and 5 respectively; the second part has 2 digits, 4 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 1135-40:
(6*1)+(5*1)+(4*3)+(3*5)+(2*4)+(1*0)=46
46 % 10 = 6
So 1135-40-6 is a valid CAS Registry Number.
InChI:InChI=1/C9H19NO3S/c11-14(12,13)8-4-7-10-9-5-2-1-3-6-9/h9-10H,1-8H2,(H,11,12,13)
1135-40-6Relevant articles and documents
Isolation of nucleic acids
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, (2008/06/13)
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
Use of zwitterionic compounds and their N-halo derivatives
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, (2008/06/13)
Zwitterionic compounds selected from:, taurine (2-aminoethanesulphonic acid), 2(N-morpholino)ethanesulphonic acid (MES), N-(2-acetamido) iminodiacetic acid (ADA), piperazine-N,N?bis(2-ethanesulphonic acid (PIPES), N-(2-acetamido)-2-aminoethanesulphonic acid (ACES), N,N-bis(2-hydroxyethyl)-2-aminoethanesulphonic acid (BES), 3-(N-morpholino)propanesulphonic (MOPS), N-N[tris(hydroxymethyl)-methyl]-2-aminoethanesulphonic acid (TES), N-2-hydroxyethylpiperazine-N?-2-ethanesulphonic acid (HEPES), N-2-hydroxyethylpiperazine-N?3-propanesulphonic acid ((H)EPPS), 2-(cyclohexylamino) ethanesulphonic acid (CHES) or 3-(cyclohexylamino) propanesulphonic acid (CAPS), and their N-halo derivatives can be used separately or in combination in the treatment of related clinical conditions by stimulating myeloperoxidase activity, which in turn stimulates hypochlorous acid production in vivo, which leads inter aliato enhanced leukotriene inactivation.