125328-76-9

Basic information

• Product Name: 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE
• Synonyms: X-1-ACETATE;5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE;1H-Indol-3-ol, 1-acetyl-5-bromo-4-chloro-;N-Acetyl-5-broMo-4-chloro-3-hydroxy-1H-indole, X-1 acetate;5-Bromo-4-chloro-3-indolyl-1-acetate;1-(5-broMo-4-chloro-3-hydroxyindol-1-yl)ethanone
• CAS NO: 125328-76-9
• Molecular Formula: C₁₀H₇BrClNO₂
• Molecular Weight: 288.53
• Product Categories: substrate
• Mol File: 125328-76-9.mol
• Article Data: 2

Chemical Properties

• Boiling Point: 423.2°C at 760 mmHg
• Flash Point: 209.8°C
• Appearance: /
• Density: 1.77g/cm³
• Vapor Pressure: 9.22E-08mmHg at 25°C
• Refractive Index: 1.674
• Storage Temp.: 2-8°C
• CAS DataBase Reference: 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE(CAS DataBase Reference)
• NIST Chemistry Reference: 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE(125328-76-9)
• EPA Substance Registry System: 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE(125328-76-9)

Safety Data

• Hazardous Substances Data: 125328-76-9(Hazardous Substances Data)

Usage

General Description

5-Bromo-4-Chloro-3-Indoxyl-1-Acetate is a chemical component often used as an intermediate for dye formulation and numerous other chemical reactions. This colorless to light yellow or white powder is also used as a substrate in enzymology, specifically for beta-glucuronidase, an enzyme that catalyzes the hydrolysis of glucuronide conjugates. When acted upon by beta-glucuronidase, it breaks down and releases a colored product that can be easily detected, making it useful for scientific research and a range of diagnostic applications. Therefore, it's important in biological assays, medical testing, as well as biochemical research and development. Its molecular formula is C10H6BrClNO3 and its safety information involves handling it with the proper protective equipment.

InChI:InChI=1/C10H7BrClNO2/c1-5(14)13-4-8(15)9-7(13)3-2-6(11)10(9)12/h2-4,15H,1H3

Upstream product

• 3030-06-6 1-acetyl-5-bromo-4-chloro-3-indolyl acetate 

Downstream Products

• 1174456-59-7 5-bromo-4-chloroindoxyl α-D-galactopyranoside 
• 29245-44-1 5,5'-dibromo-4,4'-dichloro-1H,1'H-[2,2']biindolylidene-3,3'-dione 

Relevant articles and documents

Synthesis of a 1,2-: Cis -indoxyl galactoside as a chromogenic glycosidase substrate

A synthetically challenging 1,2-cis-indoxyl galactoside, X-α-galactoside, was first prepared in this study using a cyclic ketone indoxyl acceptor and a glycosyl trichloroacetimidate donor to produce an enol glycoside and a 4,6-O-di-tert-butylsilylene-protected galactosyl donor to complete the synthesis. The target compound shows enzyme activity in the presence of α-galactosidase.