1255942-07-4 Usage
Description
DBCO-PEG4-biotin is a versatile biotinylation reagent that enables copper-free click chemistry with azide molecules to form a stable triazole linkage. This azadibenzocyclooctyne-biotin derivative is particularly useful for labeling azide-containing molecules or compounds. The PEG4 spacer increases the aqueous solubility of the molecules conjugated to the biotin compound and provides a greater distance between the reaction site with the azide and the biotin detecting group, making it more hydrophilic.
Uses
1. Activity-based protein profiling and detection of newly synthesized proteins:
DBCO-PEG4-biotin is used as a labeling agent for activity-based protein profiling and the detection of newly synthesized proteins. The application reason is its ability to form a stable triazole linkage with azide-functionalized proteins, allowing for efficient detection and analysis.
2. Immobilization of azide-labeled biomolecules:
DBCO-PEG4-biotin is used as a biotinylation reagent for the immobilization of azide-labeled biomolecules. The application reason is its copper-free click chemistry, which enables the formation of a stable triazole linkage with azide-labeled biomolecules, facilitating their immobilization on various surfaces.
3. Preparation of array-based cyclic peptide libraries:
Used in the pharmaceutical industry, DBCO-PEG4-biotin is used as a key component in the preparation of array-based cyclic peptide libraries. The application reason is its ability to react with azide-functionalized peptides, enabling the creation of diverse and complex peptide libraries for drug discovery and development.
4. General bioconjugation applications:
DBCO-PEG4-biotin is used as a bioconjugation agent in various research and diagnostic applications. The application reason is its versatility in forming stable triazole linkages with azide-containing molecules, allowing for the development of novel bioconjugates for a wide range of applications.
Check Digit Verification of cas no
The CAS Registry Mumber 1255942-07-4 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,2,5,5,9,4 and 2 respectively; the second part has 2 digits, 0 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 1255942-07:
(9*1)+(8*2)+(7*5)+(6*5)+(5*9)+(4*4)+(3*2)+(2*0)+(1*7)=164
164 % 10 = 4
So 1255942-07-4 is a valid CAS Registry Number.
1255942-07-4Relevant articles and documents
Surface functionalization using catalyst-free azide-alkyne cycloaddition
Kuzmin, Alexander,Poloukhtine, Andrei,Wolfert, Margreet A.,Popik, Vladimir V.
, p. 2076 - 2085 (2011/07/09)
The utility of catalyst-free azide-alkyne [3 + 2] cycloaddition for the immobilization of a variety of molecules onto a solid surface and microbeads was demonstrated. In this process, the surfaces are derivatized with aza-dibenzocyclooctyne (ADIBO) for the immobilization of azide-tagged substrates via a copper-free click reaction. Alternatively, ADIBO-conjugated molecules are anchored to the azide-derivatized surface. Both immobilization techniques work well in aqueous solutions and show excellent kinetics under ambient conditions. We report an efficient synthesis of aza-dibenzocyclooctyne (ADIBO), thus far the most reactive cyclooctyne in cycloaddition to azides. We also describe convenient methods for the conjugation of ADIBO with a variety of molecules directly or via a PEG linker.
