126787-65-3 Usage
Description
3-Indoxyl-beta-D-galactopyranoside, also known as Indoxyl β-D-Galactopyranoside, is a white to off-white powder with specific chemical properties. It is a synthetic compound that serves as a substrate for the enzyme beta-galactosidase and is widely utilized in various scientific applications.
Uses
Used in Microbiology Culture Media:
3-Indoxyl-beta-D-galactopyranoside is used as a selective agent for identifying lac+ organisms, such as general coliforms, in microbiology culture media. Its presence helps in the differentiation of these bacteria based on their ability to metabolize the compound, leading to a color change in the media.
Used in Histochemistry:
In the field of histochemistry, 3-Indoxyl-beta-D-galactopyranoside is employed as a vital tool for the detection and localization of beta-galactosidase activity within tissues. This application aids in the study of various cellular processes and the assessment of enzyme distribution in different cell types.
Used in Biochemistry:
3-Indoxyl-beta-D-galactopyranoside serves as a chromogenic substrate for beta-galactosidase in biochemistry. This property allows researchers to monitor the activity of the enzyme, which is crucial in various biological processes and can be indicative of certain conditions or diseases.
Used in Enzyme Assays:
As a substrate for beta-galactosidase, 3-Indoxyl-beta-D-galactopyranoside is also used in enzyme assays to measure the enzyme's activity, kinetics, and specificity. This information is valuable for understanding the enzyme's role in biological systems and for developing potential therapeutic strategies targeting beta-galactosidase.
Check Digit Verification of cas no
The CAS Registry Mumber 126787-65-3 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,2,6,7,8 and 7 respectively; the second part has 2 digits, 6 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 126787-65:
(8*1)+(7*2)+(6*6)+(5*7)+(4*8)+(3*7)+(2*6)+(1*5)=163
163 % 10 = 3
So 126787-65-3 is a valid CAS Registry Number.
InChI:InChI=1/C14H17NO6/c16-6-10-11(17)12(18)13(19)14(21-10)20-9-5-15-8-4-2-1-3-7(8)9/h1-5,10-19H,6H2/t10-,11+,12+,13-,14-/m1/s1
126787-65-3Relevant articles and documents
Improved scalable synthesis and biological activity of “indoxyl-gal,” a chromogenic histochemical used for the identification of lac+ organisms
Banasik, Brent,Mai, Tam,Samadpour, Mansour
, p. 822 - 825 (2018/11/06)
An improved and operationally simple synthesis of 3-indoxyl-β-D-galactopyranoside (in-doxyl-gal) is disclosed. Indoxyl-gal is a sensitive chromogenic histochemical used for the identification of β-galactosidase enzymatic activity (lacZ gene). Synthesis of indoxyl-gal is improved to 25% overall yield in 5 linear steps from affordable starting materials with minimal chromatography. Biological testing confirmed the detection of β-galactosidase activity in all (100%, 19 strains) coliform bacteria using membrane filtration and modified MI agar.
