1750-12-5 Usage
Description
4-Amino-3-hydrazino-1,2,4-triazol-5-thiol, also known as Purpald, is an organic compound with the chemical formula C3H6N6S. It is a beige solid that is commonly used as a reagent in various analytical and biochemical applications due to its unique chemical properties.
Uses
1. Analytical Chemistry:
4-Amino-3-hydrazino-1,2,4-triazol-5-thiol is used as a reagent for the determination of aldehydes and other reactive chemicals. It is particularly useful in the detection and quantification of these compounds due to its ability to form stable complexes with them.
2. Bacterial Polysaccharide Analysis:
In the Purpald assay, 4-Amino-3-hydrazino-1,2,4-triazol-5-thiol is used for the determination of bacterial polysaccharides containing substituted or unsubstituted glycol in residues such as glycerol, ribitol, arabinitol, furanosyl galactose, and sialic acid. It serves as a chromogen, providing a colorimetric signal that can be measured and used to quantify the presence of these polysaccharides.
3. Enzyme Activity Determination:
4-Amino-3-hydrazino-1,2,4-triazol-5-thiol is used as a chromogen during the determination of catalase activity in small tissue samples. Catalase is an enzyme that breaks down hydrogen peroxide, and the reaction with Purpald allows for the quantification of catalase activity.
4. Formaldehyde Determination:
4-Amino-3-hydrazino-1,2,4-triazol-5-thiol is used as a reagent in the determination of formaldehyde. It reacts with formaldehyde to form a colored complex, which can be measured and used to quantify the amount of formaldehyde present in a sample.
5. High-Performance Liquid Chromatography (HPLC):
In HPLC, 4-Amino-3-hydrazino-1,2,4-triazol-5-thiol is used for the derivatization of neutral monosaccharides. This process involves the chemical modification of the monosaccharides to improve their detection and separation during chromatographic analysis.
Synthesis Reference(s)
Journal of Heterocyclic Chemistry, 26, p. 355, 1989 DOI: 10.1002/jhet.5570260216
Purification Methods
Recrystallise Purpald from H2O (0.6g in 300-400mL). The benzylidene derivative has m 245-246o(dec) from i-PrOH [Hoggarth J Chem Soc 4817 1952, Dickinson & Jacobson J Chem Soc, Perkin Trans I 975 1975.[Beilstein 26 III/IV 547.]
Check Digit Verification of cas no
The CAS Registry Mumber 1750-12-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,7,5 and 0 respectively; the second part has 2 digits, 1 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 1750-12:
(6*1)+(5*7)+(4*5)+(3*0)+(2*1)+(1*2)=65
65 % 10 = 5
So 1750-12-5 is a valid CAS Registry Number.
InChI:InChI=1/C2H7N6S/c3-5-1-6-7-2(9)8(1)4/h3-4H2,(H3,5,6,7,9)
1750-12-5Relevant articles and documents
Synthesis of 3-Pyrazolyl-1,2,4-Triazoles via One-Pot Multicomponent Reaction in Phosphoric Acid
Amer, Amer Anwar,Moustafa, Amr Hassan
, p. 1703 - 1706 (2016)
A high-yielding synthetic route towards pyrazolyl-[1,2,4]triazole derivatives has been developed via one-pot multicomponent reaction of polyfunctionalized triazoles, DMF-DMA, acetophenone, ethyl cyanoacetate, and/or 3-oxo-3-phenylpropanenitrile in ortho-phosphoric acid (85%). In the same manner, a new series of 2-triazolyl-tetrahydro-indazol-4-ones and pyrazolo[3,4-d]pyrimidines has been synthesized by reaction of triazoles, DMF-DMA and 1,3-cyclohexanediones and/or barbituric acid, respectively. The same products were prepared a classical manner via reaction of triazoles with the corresponding enaminones in ortho-phosphoric acid (85%).
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Sinegibskaya et al.
, (1973)
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One-pot, Multicomponent Cascade Reaction for the Synthesis of Various Aralkyl/alkylthio-3,5-dimethyl-1H-pyrazolyl-4H-1,2,4-triazol-4-amine and Their Docking Studies
Jilloju, Parameshwara Chary,Vinaykumar, Allam,Shyam, Perugu,Vedula, Rajeswar Rao
, p. 1012 - 1019 (2019)
A facile and simple one-pot procedure for the synthesis of various aralkyl/alkylthio-3,5-dimethyl-1H-pyrazolyl-4H-1,2,4-triazol-4-amines has been described via a multicomponent reaction of 4-amino-5-hydrazinyl-4H-1,2,4-triazole-3-thiol, acetylacetone, and various aryl/alkyl halides in good yields. All the newly synthesized compounds were characterized by using analytical and spectral studies. Our in silico studies confirmed that 4e, 4f, 4g, and 4j have the best inhibition activity among the synthesized compounds with a high selective index against the Tubulin protein and showed best interactions with receptor structure. The present study provides a novel series of compounds with a promising inhibitor to prevent on Tubulin protein.
Synthesis and Characterisation of Copper(II) Complexes of 4-Amino-3-hydrazino-5-mercapto-1,2,4-triazole
Varma, R. C.,Varma, Kanchan,Verma, R. K.,Bhattacharjee, N. C.
, p. 577 - 578 (2007/10/02)
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