178925-43-4

Basic information

• Product Name: 5-BROMO-DC CEP
• Synonyms: 5'-O-(4,4'-DIMETHOXYTRITYL)-5-BROMO-N4-BENZOYLDEOXYCYTIDINE-3'-(2-CYANOETHYL-N,N-DIISOPROPYL)PHOSPHORAMIDITE;5-BROMO-DC CEP;5''-O-(4,4''-Dimethoxytrityl)-5-bromo-N4-benzoyldeoxycytidine-3''-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite;N4-Benzoyl-5-bromo-2'-deoxy-5'-O-DMT-cytidine 3'-CE phosphoramidite
• CAS NO: 178925-43-4
• Molecular Formula: C₄₆H₅₁BrN₅O₈P
• Molecular Weight: 912.8
• Mol File: 178925-43-4.mol
• Article Data: 1

Chemical Properties

• Appearance: /
• Storage Temp.: Store at -20°C
• CAS DataBase Reference: 5-BROMO-DC CEP(CAS DataBase Reference)
• NIST Chemistry Reference: 5-BROMO-DC CEP(178925-43-4)
• EPA Substance Registry System: 5-BROMO-DC CEP(178925-43-4)

Safety Data

• Hazardous Substances Data: 178925-43-4(Hazardous Substances Data)

Usage

General Description

5-BROMO-DC CEP is a chemical compound that belongs to the class of 5-Bromo-2',7'-dichlorodihydrofluorescein diacetate (Bromo-DCFDA) analogs. It is commonly used as a fluorescent probe for measuring reactive oxygen species (ROS) levels in living cells. 5-BROMO-DC CEP is an important tool in biomedical research for studying oxidative stress and oxidative damage in cells and tissues. Its fluorescence properties make it a valuable tool for detecting and quantifying ROS production in various biological systems, and it has been widely used in studies related to oxidative stress, redox signaling, and antioxidant therapies. Overall, 5-BROMO-DC CEP is a crucial chemical for understanding the role of ROS in health and disease.

Upstream product

• 951-77-9 2'-Deoxycytidine 
• 1022-79-3 5-bromo-2'-deoxycytidine 
• 100360-44-9 4-N-benzoyl-5-bromo-5'-O-(4,4'-dimethoxytrityl)-deoxycytidine 
• 102691-36-1 N,N,N',N'-tetraisopropyl 2-cyanoethylphosphorodiamidite 
• 148716-10-3 4-N-benzoyl-5-bromodeoxycytidine 

Relevant articles and documents

DNA duplexes and triplex-forming oligodeoxynucleotides incorporating modified nucleosides forming stable and selective triplexes

We have previously reported DNA triplexes containing the unnatural base triad G-PPI·C3, in which PPI is an indole-fused cytosine derivative incorporated into DNA duplexes and C3 is an abasic site in triplex-forming oligonucleotides (TFOs) introduced by a propylene linker. In this study, we developed a new unnatural base triad A-ψ·CR1 where ψ and CR1 are base moieties 2′-deoxypseudouridine and 5-substituted deoxycytidine, respectively. We examined several electron-withdrawing substituents for R1 and found that 5-bromocytosine (C Br) could selectively recognize ψ. In addition, we developed a new PPI derivative, PPIMe, having a methyl group on the indole ring in order to achieve selective triplex formation between DNA duplexes incorporating various Watson-Crick base pairs, such as T-A, C-G, A-ψ, and G-PPIMe, and TFOs containing T, C, CBr, and C3. We studied the selective triplex formation between these duplexes and TFOs using UV-melting and gel mobility shift assays.