1866-15-5 Usage
Description
Acetylthiocholine iodide is a white crystalline powder that functions as an acetylcholine receptor agonist and a substrate for acetylcholinesterase. It plays a crucial role in the study and analysis of acetylcholinesterase activity, which is essential for understanding the cholinergic system and its implications in various physiological and pathological processes.
Uses
Used in Pharmaceutical and Biomedical Research:
Acetylthiocholine iodide is used as a substrate for the colorimetric determination of acetylcholinesterase activity. This application is vital for assessing the functionality of the enzyme and its role in the regulation of neurotransmitter release and synaptic transmission.
Used in Neurochemistry and Enzymology:
Acetylthiocholine iodide serves as a substrate in the preparation of acetylcholine esterase (AChE) assay working solution for AChE activity assay. This allows researchers to evaluate the enzyme's activity and its potential as a therapeutic target for various neurological disorders, such as Alzheimer's disease and myasthenia gravis.
Used in Quality Control and Diagnostics:
In the pharmaceutical industry, acetylthiocholine iodide is utilized for quality control purposes, ensuring the efficacy and safety of drugs targeting the cholinergic system. Additionally, it is employed in diagnostic assays to detect and monitor conditions related to acetylcholinesterase dysfunction.
Used in Educational Settings:
Acetylthiocholine iodide is also used as a teaching tool in educational institutions, helping students understand the biochemical processes and mechanisms involved in neurotransmission and the role of acetylcholinesterase in the nervous system.
Purification Methods
Recrystallise the iodide from propan-1-ol (or iso-PrOH, or EtOH/Et2O) until almost colourless and dry it in a vacuum desiccator over P2O5. Its solubility in H2O is 1% w/v. A 0.075M (21.7mg/mL) solution in 0.1M phosphate buffer pH 8.0 is stable for 10-15 days if kept refrigerated. Store it away from light. It is commercially available as a 1% solution in H2O. [Ellman et al. Biochemical Pharmacology 7, 88 1961, IR: Hansen Acta Chem Scand 13 151 1959, 11 537 1957, Clin Chim Acta 2 316 1957, Ivin Zh Obshch Khim 22 267 1952, Beilstein 4 III 726, 4 IV 1585.]
Check Digit Verification of cas no
The CAS Registry Mumber 1866-15-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,8,6 and 6 respectively; the second part has 2 digits, 1 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 1866-15:
(6*1)+(5*8)+(4*6)+(3*6)+(2*1)+(1*5)=95
95 % 10 = 5
So 1866-15-5 is a valid CAS Registry Number.
InChI:InChI=1/C7H16NOS.HI/c1-7(9)10-6-5-8(2,3)4;/h5-6H2,1-4H3;1H/q+1;/p-1
1866-15-5Relevant articles and documents
Process method for preparing acetylthiocholine iodide
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Paragraph 0042; 0048-0051; 0061; 0067-0070, (2020/07/12)
The invention discloses a process method for preparing acetylthiocholine iodide, which comprises the following steps: 1) reacting potassium thioacetate used as a raw material with 1,2-dichloroethane under the action of a catalyst to obtain 2-chloroethane thioacetate; 2) reacting the 2-chloroethane thioacetate as a raw material with iodized salt to obtain 2-iodoethane thioacetate; and 3) reacting the 2-iodoethane thioacetate as a raw material with a trimethylamine compound to obtain the acetylthiocholine iodide. The method is high in safety, mild in reaction condition and high in reaction yield.
