215868-31-8 Usage
Description
Pacific Blue, also known as 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylic Acid, is a fluorescent dye that exhibits strong absorption and emission in the blue region of the spectrum. It is characterized by its high quantum yield, photostability, and water solubility, making it a versatile tool in various applications.
Uses
Used in Biomedical Research:
Pacific Blue is used as a fluorescent label for cellular and molecular biology applications, allowing for the detection and tracking of specific biomolecules, such as proteins and nucleic acids, within biological samples.
Used in Flow Cytometry:
In the field of flow cytometry, Pacific Blue is employed as a vital stain for the identification and quantification of cell surface markers, enabling researchers to analyze immune cell populations and other cellular components.
Used in Immunophenotyping:
Pacific Blue is utilized as a marker in immunophenotyping, a technique used to characterize the surface antigens of cells, particularly in the context of immune cell analysis and cancer research.
Used in Anti-idiotypic Antibody Studies:
Pacific Blue is used as a fluorescent probe in the study of anti-idiotypic antibodies targeting anti-CD19 chimeric antigen receptors derived from 4G9 scFv. This allows for the accurate detection of CAR expression on engineered T cells, which are used in adoptive immunotherapy for cancer treatment.
Used in Drug Delivery Systems:
Although not explicitly mentioned in the provided materials, Pacific Blue could potentially be used in drug delivery systems as a fluorescent marker to track the distribution and release of therapeutic agents within the body.
Check Digit Verification of cas no
The CAS Registry Mumber 215868-31-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,1,5,8,6 and 8 respectively; the second part has 2 digits, 3 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 215868-31:
(8*2)+(7*1)+(6*5)+(5*8)+(4*6)+(3*8)+(2*3)+(1*1)=148
148 % 10 = 8
So 215868-31-8 is a valid CAS Registry Number.
215868-31-8Relevant articles and documents
Hydrolysis of 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue) succinimidyl ester under acidic and basic conditions
Stockton, Amanda M.,Mora, Maria F.,Cable, Morgan L.,Willis, Peter A.,Davenport, Timothy C.,Tilley, T. Don
, p. 148 - 151,4 (2013)
The highly sensitive technique of microchip capillary electrophoresis (μCE) with laser-induced fluorescence (LIF) detection is under development for future in situ spaceflight missions to search for the organic chemical signatures of life. One fluorescent probe that enables this technology for amine, amino acid, and dipeptide analysis is 3-carboxy-6,8-difluoro-7- hydroxycoumarin (Pacific Blue, PB) succinimidyl ester. Of particular importance is the hydrolysis of PB succinimidyl ester, which precludes long-term aqueous storage during spaceflight and therefore has a significant impact on instrument design and operation. As such, it is important to characterize the chemical stability of this dye to hydrolysis prior to spaceflight. Here, we study the hydrolysis kinetics of the PB succinimidyl ester at pH values between 3 and 10.5 using μCE-LIF. The PB succinimidyl ester has the longest lifetime at pH 4 (7.3 ± 0.1 h), with dramatically shorter half-lives in the basic pH regime. This work represents a first step in the full characterization of this fluorescent probe for spaceflight applications.
Derivatives of 6,8-difluoro-7-hydroxycoumarin
-
, (2008/06/13)
The present invention comprises 6,8-difluoro-7-hydroxycoumarins and derivatives of 6,8-difluoro-7-hydroxycoumarins, including reactive dyes, dye-conjugates and enzyme substrates. These fluorine-substituted fluorescent dyes typically possess greater photostability and lower pH sensitivity in the physiological pH range than their nonfluorinated analogs, exhibit less fluorescence quenching when conjugated to a substance, possess absorption and emission spectra that closely match those of their nonfluorinated analogs, and also exhibit higher quantum yields than their nonfluorinated analogs.
