4178-93-2 Usage
Description
H-LEU-PNA, also known as L-Leucine-4-nitroanilide, is a synthetic compound commonly used as a substrate and inhibitor in various biochemical and pharmaceutical applications. It is a crystalline substance that exhibits specific interactions with enzymes and proteins, making it a valuable tool in the study and development of targeted therapies.
Uses
Used in Biochemical Research:
H-LEU-PNA is used as a substrate for the colorimetric determination of leucine aminopeptidase, an enzyme that plays a crucial role in the breakdown of proteins. This application allows researchers to study the activity and regulation of leucine aminopeptidase, which can be useful in understanding its role in various biological processes and potential therapeutic targets.
Used in Pharmaceutical Development:
As an inhibitor, H-LEU-PNA is employed in the development of drugs targeting specific enzymes or proteins. By blocking the activity of these enzymes or proteins, H-LEU-PNA can help researchers understand their function and potential as therapeutic targets for various diseases. This application is particularly relevant in the development of targeted therapies for conditions such as cancer, where inhibiting specific enzymes or proteins can help slow down or stop tumor growth.
Used in Drug Delivery Systems:
H-LEU-PNA's crystalline nature and specific interactions with enzymes and proteins make it a promising candidate for use in drug delivery systems. By incorporating H-LEU-PNA into these systems, researchers can potentially improve the delivery, bioavailability, and therapeutic outcomes of various drugs, particularly those targeting specific enzymes or proteins.
Check Digit Verification of cas no
The CAS Registry Mumber 4178-93-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,1,7 and 8 respectively; the second part has 2 digits, 9 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 4178-93:
(6*4)+(5*1)+(4*7)+(3*8)+(2*9)+(1*3)=102
102 % 10 = 2
So 4178-93-2 is a valid CAS Registry Number.
InChI:InChI=1/C12H17N3O3/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18/h3-6,8,11H,7,13H2,1-2H3,(H,14,16)/p+1/t11-/m0/s1
4178-93-2Relevant articles and documents
Kinetic study on conformational effect in hydrolysis of p-nitroanilides catalyzed by α-chymotrypsin
Kawai, Yasushi,Matsuo, Takashi,Ohno, Atsuyoshi
, p. 887 - 891 (2007/10/03)
Effects of medium viscosity on kinetics for the hydrolysis of p-nitroanilides of certain amino acid derivatives catalyzed by α-chymotrypsin have been investigated. Observed data indicate that the overall rate constant, kcat, is hardly affected by the medium viscosity in all the substrates employed and equals the rate constant at the acylation step, k2, in the measured range of viscosity. By comparison with the data on p-nitrophenyl ester substrates reported previously, it is concluded that the formation of the tetrahedral intermediate in the course of the acylation of the enzyme is influenced by conformational change of the enzyme, whereas the breakdown of the intermediate is almost free from conformational effects.
Synthesis of chromogenic substrates specific for human spleen fibrinolytic proteinase (SFP) and human leukocyte elastase (LE).
Okada,Tsuda,Hirata,Nagamatsu,Okamoto
, p. 4060 - 4068 (2007/10/02)
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