45985-24-8Relevant articles and documents
Method for preparing hindered amine nitroxide free radical compound by alkaline heterogeneous catalysis system
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Paragraph 0042-0045; 0058-0059, (2021/09/26)
The method comprises the following steps: dissolving a hindered amine compound in an organic solvent; adjusting pH by a carbonate aqueous solution; reacting with an aqueous hydrogen peroxide solution; and generating a hindered amine nitroxide free radical compound (IV). (V) Or (VI). The method is high in universality, and the hindered amine nitroxide free radical compound with various structures is prepared. The method is high in catalytic activity, short in reaction time, high in yield, simple in preparation process and convenient to operate; a high-purity target product can be obtained through simple phase separation, drying and concentration in the post-treatment process; meanwhile, the aqueous solution system and ethyl acetate can be recycled. Small by-products.
Synthesis and evaluation of β-galactosidase-targeting spin-label probe: 5-o-β-d-galactosyl-5-hydroxy-1,1,3,3-tetramethylisoindoline-2-oxyl
Ito, Tomohiro,Konno, Hiroyuki,Sato, Shingo,Sugawara, Koya
, p. 1799 - 1815 (2020/11/19)
In this study, 3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl, and 5-hydroxy-1,1,3,3-tetra-methylisoindoline-2-oxyl O-galactosides (PG, TG, and IG) were synthesized. Moreover, their reduction reactivi
2′-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
Haugland, Marius M.,El-Sagheer, Afaf H.,Porter, Rachel J.,Pe?a, Javier,Brown, Tom,Anderson, Edward A.,Lovett, Janet E.
, p. 9069 - 9072 (2016/08/05)
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.