473260-09-2Relevant articles and documents
Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition
Graham, Duncan,Grondin, Antonio,McHugh, Callum,Fruk, Ljiljana,Smith, W.Ewen
, p. 4785 - 4788 (2002)
A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diels-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2′-deoxyuridine modified at the 5-position with a spaced fura