55804-67-6Relevant articles and documents
Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells
Duan, Tingting,He, Rong,Wang, Fangwu,Wang, Xiaofeng,Xu, Zhencai,Zhou, Xuejun
, p. 24669 - 24672 (2021)
Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, a novel coumarin-based fluorescent probe, Cou-HOCl, was prepared for the detection of HOCl. The probe exhibited good selectivity over other analytes, excellent sensitivity with a detection limit of 16 nM, and fast response within 5 s. And further study demonstrated that the probe could be used not only to image exogenous HOCl in various cells, but also to determine the fluctuating levels of HOCl in macrophage cells during inflammation.
Amplification-free detection of circulating microRNA biomarkers from body fluids based on fluorogenic oligonucleotide-templated reaction between engineered peptide nucleic acid probes: Application to prostate cancer diagnosis
Metcalf, Gavin A. D.,Shibakawa, Akifumi,Patel, Hinesh,Sita-Lumsden, Ailsa,Zivi, Andrea,Rama, Nona,Bevan, Charlotte L.,Ladame, Sylvain
, p. 8091 - 8098 (2016)
Highly abundant in cells, microRNAs (or miRs) play a key role as regulators of gene expression. A proportion of them are also detectable in biofluids making them ideal noninvasive biomarkers for pathologies in which miR levels are aberrantly expressed, such as cancer. Peptide nucleic acids (PNAs) are engineered uncharged oligonucleotide analogues capable of hybridizing to complementary nucleic acids with high affinity and high specificity. Herein, novel PNA-based fluorogenic biosensors have been designed and synthesized that target miR biomarkers for prostate cancer (PCa). The sensing strategy is based on oligonucleotide-templated reactions where the only miR of interest serves as a matrix to catalyze an otherwise highly unfavorable fluorogenic reaction. Validated in vitro using synthetic RNAs, these newly developed biosensors were then shown to detect endogenous concentrations of miR in human blood samples without the need for any amplification step and with minimal sample processing. This low-cost, quantitative, and versatile sensing technology has been technically validated using gold-standard RT-qPCR. Compared to RT-qPCR however, this enzyme-free, isothermal blood test is amenable to incorporation into low-cost portable devices and could therefore be suitable for widespread public screening.
Micromolecular fluorescent probe for detecting hypochlorous acid, and preparation method and application thereof
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Paragraph 0009; 0035-0038, (2020/12/14)
The invention discloses a micromolecular fluorescent probe for detecting hypochlorous acid, and a preparation method and application thereof, and relates to the technical field of biological detection. The preparation method of the fluorescent probe comprises the following steps: reacting 8-hydroxy-nonalonidin-9-formaldehyde represented by formula (II) with ethyl acetate and piperidine in an alcohol to obtain a coumarin derivative represented by formula (III); and reacting the coumarin derivative of the formula (III) with methanesulfonic acid and 2-mercaptoethanol in an organic solution in aninert atmosphere to obtain a compound represented by formula (I), namely the fluorescent probe disclosed by the invention. The fluorescent probe provided by the invention has very high selectivity andsensitivity, and can be used for detecting the exogenous hypochlorous acid of living cells and fluorescence imaging of HOCl dynamic change in inflammatory cells as the micromolecular fluorescent probe for detecting the hypochlorous acid.
