55804-65-4Relevant articles and documents
A novel fluorescent probe with extremely low background fluorescence for sensing hypochlorite in zebrafish
Dai, Xiaoting,Dong, Lianghuan,Han, Zhixiang,Jiang, Shu,Long, Lingliang,Sun, Fan,Zhang, Min
, (2020)
Development of an efficient fluorescent probe for sensing hypochlorite in water samples and biological samples is highly demanded. However, the currently reported fluorescent probes for hypochlorite frequently suffered from the problem of high background fluorescence. Herein, based on the combined effect of two different fluorescence quenching groups, we rationally developed a novel fluorescent probe for hypochlorite with extremely low background fluorescence. Notably, due to the doubly quenching groups, the probe could even keep low background fluorescence in a solution with high viscosity. Furthermore, the probe displayed highly sensitive and selective response to hypochlorite, with the detection limits calculated to be 10.5 nM. Practical application demonstrated that the probe was able to quantitatively detect hypochlorite in various water samples with good recovery. Significantly, the probe showed extremely low background fluorescence in living cells and was capable of detecting minor variation of endogenous hypochlorite in RAW 264.7 cells. Moreover, the fluorescence imaging different concentration of hypochlorite in zebrafish has been successfully conducted. The probe developed herein will be widely used as a reliable tool to accurately monitor the variation of hypochlorite in living organism.
Convenient Retinoid X Receptor Binding Assay Based on Fluorescence Change of the Antagonist NEt-C343
Yukawa-Takamatsu, Kayo,Wang, Yifei,Watanabe, Masaki,Takamura, Yuta,Fujihara, Michiko,Nakamura-Nakayama, Mariko,Yamada, Shoya,Kikuzawa, Shota,Makishima, Makoto,Kawasaki, Mayu,Ito, Sohei,Nakano, Shogo,Kakuta, Hiroki
, p. 861 - 870 (2021)
Retinoid X receptor (RXR) modulators (rexinoids) are considered to have therapeutic potential for multiple diseases, such as Alzheimer's disease and Parkinson's disease. To overcome various disadvantages of prior screening methods, we previously developed an RXR binding assay using a fluorescent RXR ligand, CU-6PMN (4). However, this ligand binds not only at the ligand-binding domain (LBD) but also at the dimer-dimer interface of hRXRα. Here, we present a new fluorescent RXR antagonist 6-[N-ethyl-N-(5-isobutoxy-4-isopropyl-2-(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinoline-10-carboxamido)phenyl)amino]nicotinic acid (NEt-C343, 7), which emits strong fluorescence only when bound to the RXR-LBD. It allows us to perform a rapid, simple, and nonhazardous binding assay that does not require bound/free separation and uses a standard plate reader. The obtained Ki values of known compounds were correlated with the Ki values obtained using the standard [3H]9cis-retinoic acid assay. This assay should be useful for drug discovery as well as for research on endocrine disruptors, functional foods, and natural products.
A hepatocyte-targeting near-infrared ratiometric fluorescent probe for monitoring peroxynitrite during drug-induced hepatotoxicity and its remediation
Fei, Junjie,Jiang, Wen-Li,Li, Chun-Yan,Li, Yongfei,Wang, Wen-Xin,Zhao, Yi-Ting
, p. 14307 - 14310 (2019)
A novel hepatocyte-targeting near-infrared fluorescent probe named Gal-NIR is developed. Gal-NIR shows ratiometric response to ONOO- with high sensitivity and selectivity. Moreover, the probe can accurately target the hepatocyte, and thus is used for assessing drug-induced hepatotoxicity and its remediation by using hepatoprotective medicines in living cells and mice.
A PET-based fluorescent probe for monitoring labile Fe(ii) pools in macrophage activations and ferroptosis
Abedi, Syed Ali Abbas,Liu, Xiaogang,Lou, Kaiyan,Ma, Huijuan,Wang, Shanshan,Wang, Wei,Xing, Wanjin,Xu, Hang,Xu, Huan,Zhang, Xingchen
supporting information, p. 2979 - 2982 (2022/03/15)
A fluorescent probe (COU-LIP-1) for monitoring labile Fe(ii) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(ii)-induced reductive cleavage of the N-O bond results in a turn-on response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(ii) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.
Preparation method of high-sensitivity hypochlorite fluorescence probe with extremely low background fluorescence (by machine translation)
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Paragraph 0037-0039, (2020/06/24)
The invention belongs to the technical field of fluorescent probes, and particularly relates to a preparation method of a high-sensitivity hypochlorite fluorescence probe with extremely low background fluorescence. As the concentration of hypochlorite is increased, the -9 - fluorescence intensity of the fluorescent probe is gradually 343 enhanced 4 - at 2 8 - and as the concentration of hypochlorite is increased, the fluorescent probe can be used for detecting hypochlorite content in an actual water sample, detecting exogenous/endogenous hypochlorous acid roots and detecting hypochlorite in zebra fish 488 nm. (by machine translation)