57757-57-0Relevant articles and documents
Negative ion fragmentations of disulfide-containing crosslinking reagents are competitive with aspartic acid side-chain-induced cleavages
Calabrese, Antonio N.,Wang, Tianfang,Bowie, John H.,Pukala, Tara L.
, p. 238 - 248 (2013)
RATIONALE: It has been shown that the disulfide moiety in the chemical crosslinking reagent dithiobis(succinimidyl)propionate (DSP), which is similar in structure to the natural cystine disulfide, cleaves preferentially to the peptide backbone in the negative ion mode. However, the tandem mass (MS/MS) spectra of peptides in the negative ion mode are often dominated by products arising from low-energy, side-chain-induced processes, which may compete with any facile cross-linker fragmentations and complicate identification of chemical crosslinks in a complex mixture. METHODS: Two disulfide-containing crosslinking reagents similar to DSP, but with varying spacer arm lengths, were synthesized and the MS/MS spectra of several model peptides cross-linked with these reagents were investigated. Theoretical calculations were used to describe the energetics of the cross-linker fragmentations as well as several low-energy side-chain-induced fragmentations which compete with disulfide cleavages. RESULTS: Altering the spacer arm length of the cross-linker, such that there is one methylene group less than in DSP, results in a more facile cleavage process, whilst the opposite is true when a methylene group is added. Of the low-energy side-chain-induced fragmentations studied, only those from aspartic acid compete significantly with those of the cross-linker disulfide. CONCLUSIONS: Lowenergy cleavage processes from aspartic acid that compete with cross-linker fragmentations occur in the negative ion MS/MS spectra of the cross-linked peptides, irrespective of the spacer arm length. Other fragmentation pathways do not significantly interfere with low-energy disulfide cleavage, making the presence of additional product ions in the MS/MS spectrum diagnostic for the presence of aspartic acid. Copyright
Neuroprotective effect of gold nanoparticles composites in Parkinson's disease model
Hu, Kaikai,Chen, Xiaohui,Chen, Wuya,Zhang, Lingkun,Li, Jian,Ye, Jialin,Zhang, Yuxiao,Zhang, Li,Li, Chu-Hua,Yin, Liang,Guan, Yan-Qing
, p. 1123 - 1136 (2018)
Parkinson's disease (PD) is second most common neurodegenerative disorder worldwide. Although drugs and surgery can relieve the symptoms of PD, these therapies are incapable of fundamentally treating the disease. For PD patients, over-expression of α-synuclein (SNCA) leads to the death of dopaminergic neurons. This process can be prevented by suppressing SNCA over-expression through RNA interference. Here, we successfully synthesized gold nanoparticles (GNP) composites (CTS@GNP-pDNA-NGF) via the combination of electrostatic adsorption and photochemical immobilization, which could load plasmid DNA (pDNA) and target specific cell types. GNP was transfected into cells via endocytosis to inhibiting the apoptosis of PC12 cells and dopaminergic neurons. Simultaneously, GNP composites are also used in PD models in vivo, and it can successfully cross the blood-brain barrier by contents of GNP in the mice brain. In general, all the works demonstrated that GNP composites have good therapeutic effects for PD models in vitro and in vivo.
Fabrication of highly stable glyco-gold nanoparticles and development of a glyco-gold nanoparticle-based oriented immobilized antibody microarray for lectin (GOAL) assay
Huang, Li-De,Adak, Avijit K.,Yu, Ching-Ching,Hsiao, Wei-Chen,Lin, Hong-Jyune,Chen, Mu-Lin,Lin, Chun-Cheng
, p. 3956 - 3967 (2014)
The design of high-affinity lectin ligands is critical for enhancing the inherently weak binding affinities of monomeric carbohydrates to their binding proteins. Glyco-gold nanoparticles (glyco-AuNPs) are promising multivalent glycan displays that can confer significantly improved functional affinity of glyco-AuNPs to proteins. Here, AuNPs are functionalized with several different carbohydrates to profile lectin affinities. We demonstrate that AuNPs functionalized with mixed thiolated ligands comprising glycan (70 mol%) and an amphiphilic linker (30 mol%) provide long-term stability in solutions containing high concentrations of salts and proteins, with no evidence of nonspecific protein adsorption. These highly stable glyco-AuNPs enable the detection of model plant lectins such as Concanavalin A, wheat germ agglutinin, and Ricinus communis Agglutinin 120, at subnanomolar and low picomolar levels through UV/Vis spectrophotometry and dynamic light scattering, respectively. Moreover, we develop in situ glyco-AuNPs-based agglutination on an oriented immobilized antibody microarray, which permits highly sensitive lectin sensing with the naked eye. In addition, this microarray is capable of detecting lectins presented individually, in other environmental settings, or in a mixture of samples. These results indicate that glyconanoparticles represent a versatile and highly sensitive method for detecting and probing the binding of glycan to proteins, with significant implications for the construction of a variety of platforms for the development of glyconanoparticle-based biosensors.
Efficient Light-Harvesting Systems with Tunable Emission through Controlled Precipitation in Confined Nanospace
Li, Chuanqi,Zhang, Jing,Zhang, Shiyong,Zhao, Yan
supporting information, p. 1643 - 1647 (2019/01/04)
Light harvesting is a key step in photosynthesis but creation of synthetic light-harvesting systems (LHSs) with high efficiencies has been challenging. When donor and acceptor dyes with aggregation-induced emission were trapped within the interior of cross-linked reverse vesicles, LHSs were obtained readily through spontaneous hydrophobically driven aggregation of the dyes in water. Aggregation in the confined nanospace was critical to the energy transfer and the light-harvesting efficiency. The efficiency of the excitation energy transfer (EET) reached 95 % at a donor/acceptor ratio of 100:1 and the energy transfer was clearly visible even at a donor/acceptor ratio of 10 000:1. Multicolor emission was achieved simply by tuning the donor/acceptor feed ratio in the preparation and the quantum yield of white light emission from the system was 0.38, the highest reported for organic materials in water to date.
For inhibiting neural cell apoptosis of gold nano-particle complexes and use thereof
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Paragraph 0056-0058, (2018/01/19)
The invention provides a gold nanoparticle complex with an effect of inhibiting nerve cell apoptosis and an application thereof. The gold nanoparticle complex is prepared by the following steps: modifying thiolated chitosan (TCTS) onto the surface of gold nanoparticles, performing electrostatic adsorption on pDNA interfering alpha-synuclein synthesis, finally grafting a nerve growth factor by adopting a photo-grafting method, thereby obtaining the complex. The successfully synthesized gold nanoparticle complex acts on nerve cells, the cell apoptosis can be obviously inhibited by the complex, and the complex has an effect of inhibiting apoptosis of an in-vitro cell model for Parkinson's disease, is a method and novel drug for treating the Parkinson's disease occurring after operative treatment and chemotherapy and has high guiding significance for treating and researching the disease.
